Identifikasi komponen ekstrak sirih (Piper betle Linn) dari beberapa pelarut dan pemanfaatannyauntuk pengawetan ikan=Identification of betle leaf (Piper betle Linn) extracts components obtained from several solvents and

ABSTRAK The inhibition ability of betle leaf extract against the growth of spoilage bacteria (Pseudomonas fluorescens) on fish had been studied. The objectives of this research were (1) to analyze compounds of betle leaf extracted by ethanol, diethyl ether, hexane,using GC-MS, (2) to determine antibacterial activity of four kinds betle leaf extract, (3) and to use betle extracts for fish preservation. The betle leaf extracts consisted of several compounds as analysed by GC-MS. The major components were 4-allyl-l,2- diacetoxy benzene and benzoic acid derivats such as 2,4-dimethyl,benzoic acid and 3,5-dimethyl, benzoic acid. The other components were derivats of phenol and terpen. In the antibacteria activity test, betle leaf extracted with etanol showed the highest antibacteria activity, followed by diethyl eter, hexane, and aquadest extracts. Concentration below 0,1%, did not inhibit P. fluorescens growth. The inhibition of betle leaf extract againts P. fluorescens in nutrien broth (NB) with initial population of 106 CFU/ml was effective at 0,2%. Fish soaked with betle leaf extract (2%) solution for 30 minutes and during cold storage had lower aerobic plate count and pseudo-monads number as compared to control. The number of pseudomonads decreased approximately 1 log cycle after soaking in leaf extract solution for 30 minutes (8,9.10* cfu/g to 6,0.103 cfu/g), and total bacteria from 4,0.10s to 2,34.104 cfu/g. In the second day, the number of total bacteria of fish without soaking in extract solution reached 107 cfu/g, but fish soaked in extract solution for 30 minutes reached total bacteria count of 107 cfu/g after 5 days. The results indicated that betle leaf extract had the ability to extend the shelf-life of fish in cold storage. Keywords : be tie leaf-- antibacteria -fish