Endogenous biotin or biotinylated protein binding activity is a major drawback to biotin-avidin/streptavidin detection system. The avidin/streptavidin conjugate used to detect the complex of the biotinylated secondary antibody and the primary antibody binds to endogenous biotin or biotinylated proteins leading to non-specific signals. In Western blot, the endogenous biotin or biotinylated protein binding activity is usually manifested in the form of ~72kDa, ~75kDa and ~150kDa protein bands, which often mask the signals of interest. To overcome this problem, a method based on prior hybridisation of the biotinylated secondary antibody and the streptavidin conjugate was developed. The method was tested alongside the conventional biotin-strepta...
SummaryWe have developed and implemented an in vitro compartmentalization (IVC) selection scheme for...
Previous studies have shown that pretargeting protocols, using cancer-targeting fusion proteins, com...
<p>Two samples of a mouse anti-human interferon-gamma mIgG1 mAb were biotinylated using either a dir...
Biotinylation is widely used in DNA, RNA and protein probing assays as this molecule has generally n...
Exploitation of the (strept)avidin–biotin interaction is extremely valuable in a variety of biotechn...
Biotinylation is widely used in DNA, RNA and protein probing assays as this molecule has generally n...
AbstractThe sequence of the VH gene of a monoclonal anti-biotin antibody was determined. Biotin-bind...
Detection of protein-protein interactions in cells is crucial for understanding the biological funct...
Protein-protein interactions are the molecular basis of cell signaling. Recently, proximity based bi...
Disulfide links of the IgG2ak anti-ovarian carcinoma antibody, 5G6.4, were site-specifically biotiny...
Background: Chromatin immunoprecipitation (ChIP) assays coupled to genome arrays (Chip-onchip) or ma...
Müller J, Risse JM, Friehs K, Flaschel E. Model-based development of an assay for the rapid detectio...
Using biotinylated targets for detection by enzyme-linked avidin allows immunodetection methods to b...
Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was...
In diagnostic pathology and immunocytochemical research, immunohistochemical techniques using the st...
SummaryWe have developed and implemented an in vitro compartmentalization (IVC) selection scheme for...
Previous studies have shown that pretargeting protocols, using cancer-targeting fusion proteins, com...
<p>Two samples of a mouse anti-human interferon-gamma mIgG1 mAb were biotinylated using either a dir...
Biotinylation is widely used in DNA, RNA and protein probing assays as this molecule has generally n...
Exploitation of the (strept)avidin–biotin interaction is extremely valuable in a variety of biotechn...
Biotinylation is widely used in DNA, RNA and protein probing assays as this molecule has generally n...
AbstractThe sequence of the VH gene of a monoclonal anti-biotin antibody was determined. Biotin-bind...
Detection of protein-protein interactions in cells is crucial for understanding the biological funct...
Protein-protein interactions are the molecular basis of cell signaling. Recently, proximity based bi...
Disulfide links of the IgG2ak anti-ovarian carcinoma antibody, 5G6.4, were site-specifically biotiny...
Background: Chromatin immunoprecipitation (ChIP) assays coupled to genome arrays (Chip-onchip) or ma...
Müller J, Risse JM, Friehs K, Flaschel E. Model-based development of an assay for the rapid detectio...
Using biotinylated targets for detection by enzyme-linked avidin allows immunodetection methods to b...
Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was...
In diagnostic pathology and immunocytochemical research, immunohistochemical techniques using the st...
SummaryWe have developed and implemented an in vitro compartmentalization (IVC) selection scheme for...
Previous studies have shown that pretargeting protocols, using cancer-targeting fusion proteins, com...
<p>Two samples of a mouse anti-human interferon-gamma mIgG1 mAb were biotinylated using either a dir...