Synthesis, cytotoxic evaluation of substituted cinnamic-based 1,2,4-triazolo thiadiazoles

475-481In an attempt to find a new class of cytotoxic agents, a series of 3,6-disubstituted-[1,2,4] triazolo[3,4-b] [1,3,4]thiadiazoles have been synthesized using undecenoic acid and various cinnamic acids. The structures of the synthesized compounds have been confirmed using 1H and 13C NMR, IR and mass spectroscopy. The prepared compounds have been evaluated for their in vitro cytotoxic activities against four human cancer cell lines namely, HeLa, B16-F10, SKOV3, MCF7 and CHO-K1 Normal Cell line using MTT assay. Compounds 6a and 6h show promising activity against HeLa (IC50 value 8.92µM) and SKOV3 cell lines (IC50 value 9.43 µM). Majority of the compounds show significant activities against HeLa cell line with the IC50 values ranging from 8.92 to 13.44 μM. All the compounds show good activity against SKOV3 cell line with the IC50 values ranging from 9.43 to 19.34 μM. Majority of the compounds are non toxic towards the Chinese hamster ovary (CHO-K1) normal cell line