Effect of thidiazuron on <i>in vitro</i> regeneration potential of cotyledon and hypocotyl explants of cauliflower (<i>Brassica oleracea</i> L.var. <i>botrytis</i>)

126-132Plant regeneration studies were carried out with cotyledon and hypocotyl explants on four different combinations and concentrations of thidiazuron(TDZ),i.e., TDZ, TDZ+IAA (indole acetic acid), TDZ+NAA (naphthalene acetic acid) and TDZ+adenine, in cauliflower (Brassica oleracea var. botrytis cv. Pusa Snowball K1). Among the forty combinations tried, the maximum percent shoot regeneration was observed in hypocotyl explants (96%) on MS medium supplemented with 0.44 mg/L TDZ+0.08 mg/L IAA; whereas in case of cotyledon explants, only 64% shoot regeneration was achieved on MS medium supplemented with 0.77 mg/L TDZ+79.7 mg/Ladenine. For root regeneration, the in vitro regenerated shoots were cultured on MS medium containing different concentration of IBA (indole butyric acid). About 100% root regeneration was observed on MS medium supplemented with 0.10 mg/L IBA. The regenerated plantlets were transferred to the pots containing cocopeat and acclimatized. Thus, an efficient and reproducible plant regeneration protocol has been standardized in cauliflower cv. Pusa Snowball K1