Phylogenetic relationships among accessions of bamboos encountered in North Bengal, India based on RAPD and ISSR markers

495-503Bamboo is a nature’s wonderful gift associated with the rural people since ages. In the present study, genetic relationships among the 29 accessions of bamboo using RAPD and ISSR markers were accessed. The RAPD decamers resulted in 326 loci ranging 187-1875 bp, of which only two were monomorphic. The percentage of polymorphism was found to 99.39%. Dendrogram showed that the most of Bambusa species were close to each other, while the four accessions of Dendrocalamus and two accessions of Drepanostachyum were distantly placed from each other. Based on the similarity indices, being varieties of the same species, B. vulgaris, ‘Vittata’ and ‘Wamin’; B. multiplex, ‘Alphonse-Karr’ and ‘Rivierorum’, formed a cluster sharing a node at 88.3% and 91.1%, respectively. The ISSR primers produced 244 amplified bands, of which all were polymorphic (band size 137-2017 bp). The dendrogram was similar to RAPD analysis. Dendrogram based on the combined data sets of RAPD and ISSR showed considerable similarity with that obtained from individual RAPD and ISSR, except that in both the individual analysis, three accessions were segregated from the cluster and evolved as independent distinct clade, B. pallida being common. In the combined analysis, only B. pallida was found to diversify from the main clade.