Low Temperature Growth, Freezing Survival and Purificatiton of Cold Shock Protein of <i>Pseudomonas fluorescens </i>ATCC 13525

694-698Pseudomonas fluorescens ATCC 13525, a psychrotroph with temperature optima of 25°C was able to grow at 10 and 15 °C, both in minimal as well as in complex media after cold induction. In addition, the freezing survival of the bacterium at -5 and -20°C increased after cold induction. A major cold shock protein of this bacterium was purified in order to understand the intrinsic mechanisms of this protein in cryotolerance