Effect of alcohol on neurons of iso-cortex - A histomorphometric study

1259-1263Effect of chronic intake of alcohol and its subsequent withdrawl was studied in albino mice on the layers of neurons of the iso-cortex. Neuronal density per mm2 of section in different layers of iso-cortex was counted and compared in 3 group, of animals (control, ethanol fed and withdrawal). Qualitative changes on nissl granules of neurons and myelinated fibers were also studied. Mice fed with 10% ethanol v/v ad libitium for 6 months showed loss of nissl granules and nucleolus and discontinuity of nuclear membrane. Quantitatively, significant reduction in neuronal density (P<0.00 1) was observed in layers II+III,IV and V neurons of iso-cortex. Withdrawal of ethanol for 2 months showed continued reduction of counts of neuronal density in layer, II+III and V only whereas reversal of count was found significantly (P < 0.001) in layer IV of iso-cortex. Qualitatively, only few neurons, showed prominent nissl granules, after withdrawal of ethanol. More afferent synaptic connection in layer IV may be suggested as probable factor helping relative replenishment of neuronal count after withdrawal of alcohol.