<b>Tissue Culture</b>

297-298In vitro regeneration of the species was carried out on Murashige & Skoog’s (MS) medium using leaf and rhizome explants. On MS medium supplemented with 2mg/l NAA and 1mg/l BA, compact greenish white callus resulted from leaf explants. From leaf explants on MS + 2 mg/l 2, 4-D + 0.5mg/l BA, friable callus was obtained. The compact callus also yielded friable callus when transferred to B5 medium supplemented with 0.6mg/l 2,4-D and 0.4mg/l NAA. These calli were non-morphogenetic. Morphogenetic compact callus derived from leaf and rhizome explants differentiated shoots on MS + 1.0mg/l BA + 6mg/l KN. Good rooting response was observed when regenerated shoots were inoculated in modified white root culture medium and ¼ MS with 1.5% sucrose. Such plantlets were successfully transferred to soil after hardening, with a high rate of survival. The plants were comparable to natural population in growth and vigour [Prajapati A Hiren, Mehta R Saurabh and Subramanian RB, In vitro regeneration in C u r c u l i g o orchioides Gaertn.- An endangered medicinal herb, Phytomorphology, 2004, 54(1 & 2), 85-95]