Comparative analysis of transcription factors of insulin signaling (Insulin receptor substrate family)

24-30The aim of this study was to identify the role of insulin receptor substrates (IRS) in insulin signaling by doing sequence based comparative analysis. The sequences of IRS family (1, 2, 4, 5 & 6) were submitted to predict protein motif scan and clustal W for motif, domain identification and multiple sequence alignment. All the 5 IRS had some common motifs like amidation, Asn glycosylation, myristoylation, cAMP, casein kinase II, protein kinase C phosphorylation sites and functional domains like PTB and PH. Some functional motifs like tyrosine phosphorylation, Rho kinase binding, OGFR, PSGP were identified in IRS-1, RGD motifs in IRS-1 and 4, XYPPX motif and ECM protein domain in IRS-2, NLS motif and extension like domain in IRS-2 and 4, ascorbate cytosolic peroxidase, class II peroxidase superfamily domain, NHL and 2 octapeptide repeats and ATP_GTP_ attachment sites in IRS-4. These domains and motifs may have inducing and inhibitory effects on insulin signaling