<i>De novo </i>shoot regeneration from root cultures of<i> Garcinia indica </i>Choiss<i></i>

482-486Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 µM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 µM), IAA (5.71 µM) and kinetin (4.65 µM). It was observed that shoot length increased to1-2 cm. WPM medium supplemented with NAA (2.69-10.74 µM) and IBA (4.90 µM) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants