Add to ORKG448-455The rapid identification of bacteria in biological specimens is important for selection of a suitable antimicrobial therapy. We have designed a rapid (<8 h) diagnostic system that uses universal PCR primers to amplify a variable region of bacterial 16S & 23S ribosomal DNA, followed by reverse hybridization of the products to a panel of oligonucleotides, spotted on nylon membrane macroarray slides. Culture dependent assays were used as reference methods in the development and evaluation of this diagnostic platform. Broad range PCR primers were selected from the published literature to amplify 16S & 23S rDNA segments. Species-specific probe sequences were designed based on sequence alignment of eight different bacteria. These species i...
Abstract Background Our ultimate goal is to detect the entire human microbiome, in health and in dis...
Molecular detection and classification of the bacterial groups in a sample are relevant in several a...
<div><p>Within the paradigm of clinical infectious disease research, <em>Acinetobacter baumannii</em...
Background: Simultaneous and rapid detection of multiple foodborne bacterial pathogens is important ...
Abstract Background During the course of a bacterial infection, the rapid identification of the caus...
Broad-range real-time PCR and sequencing of the 16S rRNA gene region is a widely known method for th...
Broad-range real-time PCR and sequencing of the 16S rRNA gene region is a widely known method for th...
Rapid and accurate detection of pathogenic bacteria is important for the treatment of patients with ...
Abstract An oligonucleotide microarray hybridization system to differentiate microbial species was ...
A rapid and simple DNA labeling system has been developed for disposable microarrays and has been va...
The applicability of whole-cell hybridization for the identification of pathogenic bacteria in blood...
A DNA microarray was developed to detect bacterial genes conferring resistance to macrolides and rel...
Within the paradigm of clinical infectious disease research, Acinetobacter baumannii, Escherichia co...
Oligonucleotide probes targeting the small-subunit rRNA are commonly used to detect and quantify bac...
Pathogen identification is a crucial first defense against bioterrorism. A major emphasis of our nat...
Abstract Background Our ultimate goal is to detect the entire human microbiome, in health and in dis...
Molecular detection and classification of the bacterial groups in a sample are relevant in several a...
<div><p>Within the paradigm of clinical infectious disease research, <em>Acinetobacter baumannii</em...
Background: Simultaneous and rapid detection of multiple foodborne bacterial pathogens is important ...
Abstract Background During the course of a bacterial infection, the rapid identification of the caus...
Broad-range real-time PCR and sequencing of the 16S rRNA gene region is a widely known method for th...
Broad-range real-time PCR and sequencing of the 16S rRNA gene region is a widely known method for th...
Rapid and accurate detection of pathogenic bacteria is important for the treatment of patients with ...
Abstract An oligonucleotide microarray hybridization system to differentiate microbial species was ...
A rapid and simple DNA labeling system has been developed for disposable microarrays and has been va...
The applicability of whole-cell hybridization for the identification of pathogenic bacteria in blood...
A DNA microarray was developed to detect bacterial genes conferring resistance to macrolides and rel...
Within the paradigm of clinical infectious disease research, Acinetobacter baumannii, Escherichia co...
Oligonucleotide probes targeting the small-subunit rRNA are commonly used to detect and quantify bac...
Pathogen identification is a crucial first defense against bioterrorism. A major emphasis of our nat...
Abstract Background Our ultimate goal is to detect the entire human microbiome, in health and in dis...
Molecular detection and classification of the bacterial groups in a sample are relevant in several a...
<div><p>Within the paradigm of clinical infectious disease research, <em>Acinetobacter baumannii</em...