Add to ORKGA new spectrophotometric assay for human erythrocyte pyrimidine 5'-nucleotidase using a continuous spectrophotometric method is described. The activity is detected by measuring uracil, which is produced by a coupled assay including cytidine deaminase and uridine nucleosidase. The results obtained with the continuous spectrophotometric method have been confirmed by high pressure liquid chromatography (HPLC) analysis. This procedure, not based upon inorganic phosphate formation and avoiding the use of radioactive material, offers significant advantages with respect to commonly adopted assays
A fully automated procedure is proposed for the direct determination of 5'-nucleotidase in seru...
A simple and rapid spectrophotometric assay for the estimation of hypoxanthine-guanine phosphoribosy...
We describe a continuous, spectrophotometric, enzyme-coupled assay useful to monitor reactions catal...
A new spectrophotometric assay for human erythrocyte pyrimidine 5'-nucleotidase using a continuous s...
A micellar electrokinetic chromatographic method that allows simultaneous determination of both nucl...
ABSTRACT A simple method for determining pyrimidine 5'-nucleotidase (P5N) activity in whole blo...
A purine 5'-nucleotidase has been separated by DEAE-Trisacryl chromatography from other 5'-nucleotid...
Following an outline of the chemistry and biochemistry of the purine and pyrimidine bases and their ...
Serum 5’nucleotidase activity has been measured by a coupled kinetic assay in which adenosine formed...
We describe a fully enzymatic, one-step kinetic method, involving use of 30 4 of serum, for determin...
Summary: The continuous spectröphotometric assay of 5'-nucleotidase originally described by Hei...
We describe a multi-point method for the determination of the serum enzyme activity 5’-nucleotidase ...
AbstractTwo cytoplasmic forms of pyrimidine nucleotidase (PN-I and PN-II) have been purified from hu...
Erythrocyte maturation is accompanied by RNA degradation and release of mononucleotides. Pyrimidine ...
We present a sensitive colorimetric determination of 5’-nucleotidase based on the measurement of lib...
A fully automated procedure is proposed for the direct determination of 5'-nucleotidase in seru...
A simple and rapid spectrophotometric assay for the estimation of hypoxanthine-guanine phosphoribosy...
We describe a continuous, spectrophotometric, enzyme-coupled assay useful to monitor reactions catal...
A new spectrophotometric assay for human erythrocyte pyrimidine 5'-nucleotidase using a continuous s...
A micellar electrokinetic chromatographic method that allows simultaneous determination of both nucl...
ABSTRACT A simple method for determining pyrimidine 5'-nucleotidase (P5N) activity in whole blo...
A purine 5'-nucleotidase has been separated by DEAE-Trisacryl chromatography from other 5'-nucleotid...
Following an outline of the chemistry and biochemistry of the purine and pyrimidine bases and their ...
Serum 5’nucleotidase activity has been measured by a coupled kinetic assay in which adenosine formed...
We describe a fully enzymatic, one-step kinetic method, involving use of 30 4 of serum, for determin...
Summary: The continuous spectröphotometric assay of 5'-nucleotidase originally described by Hei...
We describe a multi-point method for the determination of the serum enzyme activity 5’-nucleotidase ...
AbstractTwo cytoplasmic forms of pyrimidine nucleotidase (PN-I and PN-II) have been purified from hu...
Erythrocyte maturation is accompanied by RNA degradation and release of mononucleotides. Pyrimidine ...
We present a sensitive colorimetric determination of 5’-nucleotidase based on the measurement of lib...
A fully automated procedure is proposed for the direct determination of 5'-nucleotidase in seru...
A simple and rapid spectrophotometric assay for the estimation of hypoxanthine-guanine phosphoribosy...
We describe a continuous, spectrophotometric, enzyme-coupled assay useful to monitor reactions catal...