Add to ORKGchain (PCR) reaction assay targeted mitochondrial DNA (mtDNA) species-specific gene. Meatballs spiked with 1.0% (w/w) and 5.0% (w/w) porcine meat and gelatin, respectively, were prepared and heat-treated using five (n=5) cooking methods: boiling, pan-frying, roasting, microwaving and autoclaving. Two pairs of mtDNA primers were targeted in short sequences using polymerase chain reaction (PCR) analysis, producing 212- and 83-bp amplicons. Electrophoresis analysis showed positive results for porcine DNA at 1.0% and 5.0% for both porcine meat and gelatin for all of the different cooking techniques. Thus, PCR analysis using species-specific primers was demonstrated to be very useful for the detection of porcine DNA in cooked meatballs
Wild boar meat (WBM) is non-halal meat widely abused in Indonesia. The most common case is mixing be...
The risk of bovine spongiform encephalopathy propagation was drastically reduced after the European ...
The presence of pork in any meat products including abon (shredded meat) is not allowed for Islamic ...
The addition of blood plasm in meatballs become an issue and concerned by Muslim’s consumers in Mala...
Food adulteration has attracted a great deal of public concern as it affects consumers of different ...
Analysis of non-halal components, such as pork and porcine gelatin, in food and pharmaceutical produ...
Meat is one of food materials which has protein source and mostlyconsumed by non-vegetarian. Consumi...
Identification the type of meat in processed meat products concern and consideration because of vari...
Point-of-care diagnostic methods for animal species determination are critical for rapid, simple, an...
Porcine contamination was one important issue in food certification for Halal. Here, we reported PCR...
The expensive beef have encouraged counterfeiting beef on processed food products such as meatballs....
A unique DNA isolation protocol based on microwave irradiation and density centrifugation for the si...
Porcine DNA authentication in commercial products is critical due to improper or non-certified Halal...
A polymerase chain reaction (PCR) assay for the assessment of dog meat adulteration in meatballs was...
Identification of meat species in processed meat products is a major problem in meat technology to p...
Wild boar meat (WBM) is non-halal meat widely abused in Indonesia. The most common case is mixing be...
The risk of bovine spongiform encephalopathy propagation was drastically reduced after the European ...
The presence of pork in any meat products including abon (shredded meat) is not allowed for Islamic ...
The addition of blood plasm in meatballs become an issue and concerned by Muslim’s consumers in Mala...
Food adulteration has attracted a great deal of public concern as it affects consumers of different ...
Analysis of non-halal components, such as pork and porcine gelatin, in food and pharmaceutical produ...
Meat is one of food materials which has protein source and mostlyconsumed by non-vegetarian. Consumi...
Identification the type of meat in processed meat products concern and consideration because of vari...
Point-of-care diagnostic methods for animal species determination are critical for rapid, simple, an...
Porcine contamination was one important issue in food certification for Halal. Here, we reported PCR...
The expensive beef have encouraged counterfeiting beef on processed food products such as meatballs....
A unique DNA isolation protocol based on microwave irradiation and density centrifugation for the si...
Porcine DNA authentication in commercial products is critical due to improper or non-certified Halal...
A polymerase chain reaction (PCR) assay for the assessment of dog meat adulteration in meatballs was...
Identification of meat species in processed meat products is a major problem in meat technology to p...
Wild boar meat (WBM) is non-halal meat widely abused in Indonesia. The most common case is mixing be...
The risk of bovine spongiform encephalopathy propagation was drastically reduced after the European ...
The presence of pork in any meat products including abon (shredded meat) is not allowed for Islamic ...