Sequence analysis of endo-?-1,3-1,4- glucanase gene (?glu) isolated from Bacillus SP 289, an antagonist bacteria against rice sheath blight pathogen

Glucanases are enzymes that function to break down a glucan, a polysaccharide made of several glucose subunits. As they perform hydrolysis of the glucosidic bond, they are hydrolases and classified as glycoside hydrolases EC.3.2 in the EC number classification of enzymes. Glycoside hydrolases are a well-known group of enzymes. Their functions are to hydrolyse the glycosidic bond between a carbohydrate and non-carbohydrate moiety or between 2 or more carbohydrates. Naturally, ?-glucanases are involved in catalyzing ?-glucan degradation. These ?-glucanases are divided into 4 type according to their glycosidic linkage that they cleavei) ?-1,3-1,4-glucanases (lichenases, EC 3.2.1.73), ii) ?- 1,3-glucanases (laminarinases, EC 3.2.1.39), iii) ?-1,4-glucanases (cellulases, EC 3.2.1.4) and iv) ?-1,3(4)- glucanases (EC 3.2.1.6) (Yang et al., 2008). (Copied from article)