Different doses of purified extract of cacao bean (Theobroma cacao) to IgM antibody profile in Wistar rat stimulated by ovalbumin antigen

Cacao bean is a rich source of polyphenol. It possesses antioxidant activity and can modulate an immune system. This study was conducted to test various doses of purified extract of cacao seeds, rich in polyphenols, to IgM antibody profile of wistar white rats on the fifth and seventh day after being stimulated by ovalbumin antigen. The production of IgM antibody on the fifth and seventh day were assayed by Elisa. The oral feeding of the purified extract at a dose of 30 mg/100 g body weight of rats did not elevate the number of IgM compared to the control group. The level of IgM production after administration of purified extract at dose of 60 mg/100 g body weight was increased significantly on the fifth day to the control group. The enhancing of the IgM level on the fifth day also was showed at the dose of 120 mg/100 g to the control group but its IgM level is less than the IgM level of the dose of 60 mg/100 g body weight. This research also presented that the amount of IgM on the seventh day decreased compared to the fifth day. The research showed that the effective treatment is at the dose of 60 mg/100 g body weight of rat