Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
- Santos, Maria Victoria
- Sansinena, Marina Julia
- Zaritzky, Noemi Elisabet
- Chirife, Jorge
DOIAbstract
A widespread practice in cryopreservation is to freeze spermatozoa by suspending the straws in stagnant nitrogen vapor over liquid nitrogen (N2V/LN2) for variable periods of time before plunging into liquid nitrogen (-196 C) for indefinite storage. A mathematical heat transfer model was developed to predict freezing times (phase change was considered) required for bull semen and extender packaged in 0.5 ml plastic straws and suspended in static liquid nitrogen vapor. Thermophysical properties (i.e. thermal conductivity, specific heat, density, initial freezing temperature) of bovine semen and extender as a function of temperature were determined considering the water change of phase. The non-stationary heat transfer partial differential equ...
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