Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
- Lacunza, Ezequiel
- Montanaro, Mauro Aldo
- Salvati, Annamaria
- Memoli, Domenico
- Rizzo, Francesca
- Henning, Maria Florencia
- Quiroga, Ivana Yoseli
- Guillou, Hervé
- Abba, Martín Carlos
- Gonzalez Baro, Maria del Rosario
- Weisz, A
- Pellon Maison, Magali
DOIAbstract
Glycerol-3-phosphate acyltransferase-2 is a member of “cancer-testis gene” family. Initially linked to lipid metabolism, this gene has been recently found involved also in PIWI-interacting RNAs biogenesis in germline stem cells. To investigate its role in piRNA metabolism in cancer, the gene was silenced in MDA-MB-231 breast cancer cells and small RNA sequencing was applied. PIWI-interacting RNAs and tRNA-derived fragments expression profiles showed changes following GPAT2 silencing. Interestingly, a marked shift in length distribution for both small RNAs was detected in GPAT2-silenced cells. Most downregulated PIWI-interacting RNAs are single copy in the genome, intragenic, hosted in snoRNAs and previously found to be upregulated in cancer...
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