Uji Kepekaan Bakteri Asam Laktat Kandidat Probiotik terhadap Antibiotik Kanamisin, Oleandomisin, dan Polimiksin B

Bakteri probiotik utama adalah strain yang berasal dari genera Lactobacillus dan Bifidobacterium.Bakteri asam laktat (BAL) sensitif terhadap antibiotik beta laktam, kecuali penisilin. Beberapa BAL sepertiLactobacillus dan Lactococcus resisten terhadap tetrasiklin dan eritromisin. Tujuan penelitian ini adalahmengetahui kepekaan bakteri asam laktat kandidat probiotik terhadap antibiotik kanamisin, oleandomisin,dan polimiksin B. Penelitian dilakukan di Bagian Mikrobiologi Klinik, Balai Besar Laboratorium Kesehatan(BBLK) Sulawesi Selatan, pada bulan Mei-September 2017. Isolat bakteri berasal dari hasil isolasi pisangkemudian dilakukan uji sensitivitas dan resistensi bakteri terhadap antibiotik kanamisin, oleandomisin, danpolimiksin menggunakan VITEK 2 Compact biomereux clinical diagnostics (USA). Hasil uji resistensi daritujuh sampel isolat bakteri asam laktat (BAL) kandidat probiotik menunjukkan tingkat sensitivitas paling tinggiterhadap antibiotik kanamisin dan oleandomisin terdapat pada tiga isolat yaitu 1C,1E, dan 5D; hanya satubakteri yang sensitif pada polimiksin B yaitu isolat 5D. Isolat bakteri asam laktat dari hasil isolasi pisang dapatdiuji lebih lanjut sebagai kandidat probiotik. Kata kunci: sensitivitas, resistensi, kanamisin, oleandomisin, polimiksin B.   Sensitivity and Resistance of Antibiotic Kanamycin, Oleandomycin andPolymyxin B on Lactid Acid Bacteria as Probiotic Candidate Abstract Probiotics are living microorganisms which, in sufficient quantities, could affect their hosts to be healthier.The main probiotic bacteria are strains originating from the genera Lactobacillus and Bifidobacterium. Thepurpose of this study was to determine the sensitivity of candidate probiotic lactic acid bacteria to kanamycin,oleandomicin, and polymixin B. Bacterial isolates originated from banana and the sensitivity and resistanceof these bacteria were then tested to kanamycin, oleandimycin, and polymyxin B antibiotics using VITEK 2Compact biomereux clinical diagnostics (USA). The resistance test results from seven samples of lactic acidbacteria isolates of probiotic candidates showed the highest level of sensitivity to kanamycin and oleandomisinantibiotics found in three isolates namely 1C, 1E, and 5D; only one bacterium that was sensitive to polymyxinB is the 5D isolate. Isolates of lactic acid bacteria can be further tested as probiotic candidates. Keywords: sensitivity, resistance, kanamycin, oleandomycin, polymyxin B.&nbsp