Gambaran Histologi Regenerasi Hati Pasca Penghentian Pajanan Monosodium Glutamat pada Tikus Wistar

Monosodium glutamat (MSG) merupakan bahan penyedap masakan yang sering digunakan, namun konsumsi MSG berlebihan dapat merusak hati. Tujuan penelitian ini adalah mengetahuipengaruh pajanan MSG terhadap gambaran histologis hati tikus jantan galur wistar dankemampuan regenerasinya. Penelitian ini menggunakan desain eksperimental post-test only.Tikus dibagi menjadi 9 kelompok, yang terdiri atas 3 kelompok perlakuan dalam 3 periode yang berbeda (28, 42, dan 56 hari). Kelompok kontrol positif 1, 2, dan 3 diberikan akuades selama 28 hari, 42 hari, 56 hari; kelompok kontrol negatif 1, 2, dan 3 diberikan MSG 5 mg/gBB/hari selama 28hari, 42 hari, 56 hari; kelompok perlakuan regenerasi 1, 2, 3 diberikan MSG 5 mg/gBB/hari selama28 hari kemudian dihentikan selama 0 hari, 14 hari, 28 hari. Variabel yang diukur adalah derajatkerusakan jaringan hati. Pada analisis one way ANOVA terdapat perbedaan signifikan (p<0,05).Uji post hoc LSD menunjukkan perbedaan signifikan antara kelompok kontrol positif dengankelompok kontrol negatif (p<0,05) dan tidak terdapat perbedaan signifikan antara kelompok kontrolpositif dengan perlakuan regenerasi 14 hari (p>0,05) dan perlakuan regenerasi 28 hari (p>0,05).Disimpulkan pajanan MSG mengakibatkan kerusakan hati dan terjadi regenerasi hati setelah 14hari penghentian pajanan MSG. Kata kunci: monosodium glutamat, regenerasi, kerusakan hati Histological Study of Liver Regeneration after Cessation ofMonosodium Glutamat on Rats Abstract Monosodium glutamat (MSG) is flavor enhancer that has been used in various food products. Excessive consumption of MSG have been reported to damage liver. The purpose of this experimentis to determine effect of MSG on male wistar rats’s liver histology and it’s regeneration capability.This is an experimental research with post-test only goup design. Rats were divided into 9 goups,consisted of 3 treatment goups with 3 different period (28, 42, and 56 days). 1 positivecontrol goup were given akuades for 28, 42, and 56 days; 1st, 2nd, and 3rdst,  negative control goup weregiven 5 mg/gBW/day of MSG for for 28, 42, and 56 days; 1st, 2nd, and 3 treatment goup were given 5mg/gBW/day of MSG for 28, 42, and 56 days, then stopped for 0, 14, and 28 days. Measured variablewere liver damage degree. One Way ANOVA data analysis found a significant difference (p<0.05)and there was no significant difference between positive control and regeneration treatment goup day 14th (p>0.05) and regeneration treatment group day 28thrd2nd3, and 3rd (p>0.05). MSG exposure causes liver damage and liver regeneration occurs after 14 days cessation of MSG exposure. Keywords: monosodium glutamate (MSG), regeneration, liver damag