NMR Spektroskopische Untersuchungen von Körperflüssigkeiten (Kinderurin)

Urine is an information-rich biofluid that can provide insights into the metabolic state of an organism. While urine is easy to gain, the investigation is difficult since every metabolite needs a single analysis. NMR offers several advantages for metabolomic investigations. It requires minimal or no sample preparation and small sample volume. It is non-destructive and allows the observation of a large number of metabolites simultaneously and without preselection. A goal of this study was the assignment of resonances of recent metabolites in the one- and two-dimensional NMR spectra of human urine since the inherent complexity of urine NMR spectra is a common barrier to apply targeted investigations. The identification of recent metabolites was assisted by using SPE and HPLC.Changes in metabolic profiles resulting from diet may be difficult to differentiate from normal or pathological physiologic variation. Thus another aim was the definition of dietary influence on the composition of urine. We examined the effect of eating banana, pecans and oranges on the composition of urine. The metabolites were quantified on the basis of peak heights and were expressed in terms of Creatinine, which can provide an internal standard