Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.
- Monaco, A
- Larin, Z
- Lehrach, H
Publication date
January 1994
DOI Publisher
Springer Science and Business Media LLC ISSN
1073-6085Abstract
Yeast artificial chromosome (YAC) libraries have been constructed from a variety of organisms using different approaches. This protocol outlines in detail the construction of YAC libraries with large inserts using size fractionation of partially digested DNA by pulsed-field gel electrophoresis
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