Context. Constraining additional mixing processes and chemical composition is a central problem in stellar physics as their impact on determining stellar age leads to biases in our studies of stellar evolution, galactic history and exoplanetary systems. In two previous papers, we have shown how seismic inversion techniques could be used to offer strong constraints on such processes by pointing out weaknesses in current theoretical models. The theoretical approach having been tested, we now wish to apply our technique to observations. In that sense, the solar analogues 16CygA and 16CygB, being amongst the best targets in the Kepler field, are probably currently the most well suited stars to test the diagnostic potential of seismic inversions...