Identification of epigenetic DNA modifications with a protein nanopore.
- Wallace, E
- Stoddart, D
- Heron, A
- Mikhailova, E
- Maglia, G
- Donohoe, T
- Bayley, H
DOIAbstract
Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA
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