Gh53 Endo-beta-1,4-galactanase From A Newly Isolated Bacillus Licheniformis Cbmai 1609 As An Enzymatic Cocktail Supplement For Biomass Saccharification

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Galactanases (endo-beta-1,4-galactanases-EC 3.2.1.89) catalyze the hydrolysis of beta-1,4 galactosidic bonds in arabinogalactan and galactan side chains found in type I rhamnogalacturan. The aim of this work was to understand the catalytic function, biophysical properties, and use of a recombinant GH53 endo-beta-1,4-galactanase for commercial cocktail supplementation. The nucleotide sequence of the endo-beta-1,4-galactanase from Bacillus licheniformis CBMAI 1609 (Bl1609Gal) was cloned and expressed in Escherichia coli, and the biochemical and biophysical properties of the enzyme were characterized. The optimum pH range and temperature of Bl1609Gal activity were 6.5-8 and 40 A degrees C, respectively. Furthermore, Bl1609Gal showed remarkable pH stability, retaining more than 75 % activity even after 24 h of incubation at pH 4-10. The enzyme was thermostable, retaining nearly 100 % activity after 1-h incubation at pH 7.0 at 25-45 A degrees C. The enzymatic efficiency (K (cat) /K (m) ) against potato galactan under optimum conditions was 241.2 s(-1) mg(-1) mL. Capillary zone electrophoresis demonstrated that the pattern of galactan hydrolysis by Bl1609Gal was consistent with that of endogalactanases. Supplementation of the commercial cocktail ACCELLERASE(A (R))1500 with recombinant Bl1609Gal increased hydrolysis of pretreated sugarcane bagasse by 25 %.1793415426CNPq [481666/2012-5]CAPESFAPESP [2013/144543]Laboratorio de Analises de Macromoleculas (LAM) at Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP