Ultrasound Associated With Caffeine Increases Basal And Beta-adrenoceptor Response In Adipocytes Isolated From Subcutaneous Adipose Tissue In Pigs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The topical use of caffeine has been indicated for the lipodystrophies treatment as it promotes increased lipolysis. Ultrasound (US) is often used in cutaneous diseases, esthetic conditions, and as a skin permeation enhancer. Objective: We investigate the lipolytic response of adipocytes isolated from subcutaneous adipose pigs tissue subjected to treatment with topical application of phonophoresis associated with caffeine. Method: We treated dorsal regions of pigs (Landrace x Large White, 35 days, 15 kg, n = 6) daily for 15 days with gel, gel + US [3 MHz, continuous, 0.2 Wcm(2), 1 min/cm(2), in total 2 min], gel + caffeine (5%w/w), and gel + caffeine + US. We used a fifth untreated region as control. Twenty-four hours after the last application, we isolated the adipocytes of each treated area and quantified the basal and stimulated lipolytic responses to isoprenaline. The results, in mu mol glycerol/10(6)cells/60 min, were analyzed with analysis of variance or ANOVA followed by Newman-Keuls test. The value of p < 0.05 was indicative of statistical difference. Results: Only the adipocytes isolated from the area treated with caffeine + US showed increased basal lipolysis (0.76 +/- 0.26; p = 0.0276) and maximal isoprenaline stimulation (0.38 +/- 0.15, p = 0.0029) compared with the other areas. Conclusion: The results demonstrate that increased lipolysis of caffeine + US is due to an increase in basal and beta-adrenoceptor response by caffeine, and caffeine's effect is local, avoiding unwanted effects.182116123University of Campinas (UNICAMP)Foundation for Research Support of the State of Sao Paulo-FAPESP, Teaching and Research Support Foundation (FAEP)University Methodist of Piracicaba (UNIMEP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP