Cellulose Nanofibers Produced From Banana Peel By Enzymatic Treatment: Study Of Process Conditions

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Cellulose nanofibers (CNFs) were isolated from banana peel bran via alkaline treatment followed by enzymatic treatment with xylanase. The influence of process conditions such as pH, temperature, and concentrations of the enzyme and substrate on the properties of the CNFs was evaluated with a 2(4-1) fractional factorial design with three central points. Enzyme at 70 Uig of bran, substrate at 15%, pH 6.0, and temperature between 35 and 55 degrees C favored enzymatic hydrolysis. Transmission electron microscopy (TEM) images confirmed that treatment with xylanase effectively isolated cellulose fibers at the nanometer scale. Fourier transform infrared spectroscopy (FTIR) showed that a fraction of amorphous compounds was removed. X-ray diffraction revealed that the CNFs presented high crystallinity index (66.2%). The CNFs had a diameter of 3.7 nm, their aspect ratio was in the range of long nanofibers, and their suspension was stable (-29.1 mV). These features make the CNFs potentially applicable as reinforcing agents in composites. The results evidenced that enzymatic hydrolysis with xylanase successfully afforded CNFs from banana peel, a residue that constitutes a potential source of biodegradable materials of commercial interest. (C) 2016 Elsevier B.V. All rights reserved.95664674Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [2952/2011]Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [477842/2011-9]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq