Presynaptic Action Of Bothriopsis Bilineata Smargadina (forest Viper) Venom In vitro.
- Rodrigues-Simioni, Léa
- Floriano, Rafael Stuani
- Rostelato-Ferreira, Sandro
- Sousa, Norma Cristina
- Marangoni, Sergio
- Ponce-Soto, Luis Alberto
- Carregari, Victor Corasolla
- Hyslop, Stephen
DOIAbstract
In this work, we examined the neuromuscular activity of Bothriopsis bilineata smargadina (forest viper) venom in vertebrate isolated nerve-muscle preparations. In chick biventer cervicis preparations the venom caused concentration-dependent (0.1-30 μg/ml) neuromuscular blockade that was not reversed by washing, with 50% blockade occurring in 15-90 min. Muscle contractures to exogenous acetylcholine and KCl were unaffected by venom, but there was a slight increase in creatine kinase release after 120 min (from 80 ± 15 to 206 ± 25U/ml, n=6, p<0.05). In mouse phrenic nerve-diaphragm preparations, the venom (1, 10 and 30 μg/ml) produced marked facilitation (∼120% increase above basal) at the highest concentration followed by neuromuscular block...
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