Add to ORKGRetroviruses must ensure that poly(A) signals in the 3' LTR are highly active, while identical signals in the 5' LTR are inactive (occluded). In the case of HIV-1, both promoter proximity in the 5' LTR and U3 sequences in the 3' LTR may contribute to this regulation. We have discovered a novel regulatory mechanism for poly(A) site occlusion in HIV-1. When transcription initiation from the HIV promoter is activated by Tat, the HIV poly(A) site is specifically occluded, while other poly(A) sites are unaffected by Tat. Nucleotide signals associated with this Tat effect are immediately adjacent to the AAUAAA sequence of the HIV-1 poly(A) signal. These data suggest that elongating RNA polymerase II, activated by Tat specifically occludes the HIV...
It has previously been shown that the human immunodeficiency virus type 1 (HIV-1) trans-activation-r...
In HIV-1 infection, Tat acts at least in part to control transcriptional elongation by overcoming pr...
Maximal gene expression in retroviruses requires that polyadenylation in the 5' long terminal repeat...
Retroviruses must ensure that poly(A) signals in the 3' LTR are highly active, while identical signa...
AbstractThe human immunodeficiency virus type 1 (HIV-1) Tat protein is a transcriptional activator t...
In common with all retroviruses, the human immunodeficiency virus type 1 (HIV-1) contains duplicated...
To investigate the selective use of poly(A) sites in the 3' long terminal repeat (LTR) but not the 5...
Transcription of the HIV-1 provirus produces a primary transcript that has terminally redundant poly...
Immediately after HIV infects a cell, the virion RNA is copied into DNA and the proviral genome is t...
The HIV-1-encoded Tat protein controls transcription elongation by increasing processivity of RNA po...
HIV-1 gene expression has provided an excellent model of transcription elongation control. Transcrip...
The 5$ sp prime$ untranslated region of human immunodeficiency virus type 1 (HIV-1) mRNAs comprises ...
We studied regulation of human immunodeficiency virus-1 (HIV-1) transcription by Tat and, for compar...
It is generally acknowledged that the Tat protein has a pivotal role in HIV-1 replication because it...
We examined the activation of transcription from the human immunodeficiency virus type 1 (HIV-1) pro...
It has previously been shown that the human immunodeficiency virus type 1 (HIV-1) trans-activation-r...
In HIV-1 infection, Tat acts at least in part to control transcriptional elongation by overcoming pr...
Maximal gene expression in retroviruses requires that polyadenylation in the 5' long terminal repeat...
Retroviruses must ensure that poly(A) signals in the 3' LTR are highly active, while identical signa...
AbstractThe human immunodeficiency virus type 1 (HIV-1) Tat protein is a transcriptional activator t...
In common with all retroviruses, the human immunodeficiency virus type 1 (HIV-1) contains duplicated...
To investigate the selective use of poly(A) sites in the 3' long terminal repeat (LTR) but not the 5...
Transcription of the HIV-1 provirus produces a primary transcript that has terminally redundant poly...
Immediately after HIV infects a cell, the virion RNA is copied into DNA and the proviral genome is t...
The HIV-1-encoded Tat protein controls transcription elongation by increasing processivity of RNA po...
HIV-1 gene expression has provided an excellent model of transcription elongation control. Transcrip...
The 5$ sp prime$ untranslated region of human immunodeficiency virus type 1 (HIV-1) mRNAs comprises ...
We studied regulation of human immunodeficiency virus-1 (HIV-1) transcription by Tat and, for compar...
It is generally acknowledged that the Tat protein has a pivotal role in HIV-1 replication because it...
We examined the activation of transcription from the human immunodeficiency virus type 1 (HIV-1) pro...
It has previously been shown that the human immunodeficiency virus type 1 (HIV-1) trans-activation-r...
In HIV-1 infection, Tat acts at least in part to control transcriptional elongation by overcoming pr...
Maximal gene expression in retroviruses requires that polyadenylation in the 5' long terminal repeat...