Preparation and Characterization of a Microwave-Immobilized Fluorinated Stationary Phase for RP-LC

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)A fluorinated stationary phase was prepared through the immobilization of poly(methyl-3,3,3-trifluoropropylsiloxane) onto 5 mu m Kromasil silica by microwave irradiation. The best conditions of immobilization time and temperature were determined by central composite design and response surface methodology. Physical-chemical characterizations (IR, (29)Si NMR and elemental analysis) confirmed that the polymer was attached onto the chromatographic support by different mechanisms that resulted in a percent carbon loading of 10%. Some pharmaceuticals were completely separated with the fluorinated stationary phase using a simple mobile phase while the same separation was not possible with a C18 stationary phase.7241858617626Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq