Whey protein coating bead improves the survival of the probiotic Lactobacillus rhamnosus CRL 1505 to low pH

Aims: To evaluate the efficacy of a novel microencapsulation procedure using whey protein and pectin to improve the survival rate of Lactobacillus rhamnosus CRL 1505 to low pH and bile. Methods and Results: Lactobacillus rhamnosus CRL 1505 was encapsulated by ionotropic gelation using pectin (PE) and pectinwhey protein (PEWP). Both types of beads (MCPE/WP and MCPEWP/WP) were covered with a layer of whey protein by complex coacervation. The noncapsulated lactobacilli were not sensitive to bile salts but to acid. Both microparticles protected Lact.similar to rhamnosus CRL 1505 at pH 2.0, but only MCPE/WP was effective at pH 1.2. Conclusions: The combination of ionotropic gelation and complex coacervation techniques is efficient to obtain microcapsules of pectin covered with whey proteins. The MCPE/WP beads were more stable than the MCPEWP/WP beads in simulated gastric conditions, thus offering better protection to Lact.similar to rhamnosus CRL 1505 at low pH. Significance and Impact of the Study: Pectin beads with a whey protein layer (MCPE/WP) could be used as probiotic carrier in functional foods of low pH (e.g. apple juice), thus protecting Lact.similar to rhamnosus CRL 1505 against the stressful conditions of the gastric tract.546552556Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), ArgentinaAgencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT), ArgentinaCIUNT, Argentin