Improvement of trypanocidal metabolites production by Aspergillus fumigatus using neural networks

An optimization procedure using artificial, neural networks was developed to determine the optimal. combination of parameters, such as medium culture, initial pH, temperature and time of fermentation for maximal. trypanocidal metabolites production by Aspergillus fumiyatus. A data set of 81 experiments was carried out and an artificial neural network was trained to identify the optimal. conditions for this process. Good correlation was obtained between the experimental. and predicted values of lysis of the trypomastigote forms of Tryponosoma cruzi (r(2) = 0.9990). The simulations of fermentation performance were undertaken on combinations of input variables and the highest Level. of activity against T cruzi was obtained from the chloroform extract of the modified Jackson medium culture, initial pH of 6.0, incubated at 40 ° C for 144 h. It displayed lysis of 95% of the trypomastigote forms of T cruzi and the red blood cells remained normal. © 2004 Elsevier GmbH. All rights reserved.160214114