Characterization of microsatellite markers for snouted treefrogs in the Scinax perpusillus species group (Anura, Hylidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)We characterized eight microsatellite loci for snouted treefrogs in the Scinax perpusillus species group, a group of hylid frogs endemic to the Atlantic Coastal Forest of Brazil, and tested their utility in mainland and island species of the complex. All eight loci were polymorphic in one population of S. perpusillus; four of the loci showed excess homozygosity and three of those deviated from Hardy-Weinberg expectations, possibly due to null alleles, inbreeding, or population structure in sampled individuals. Six loci amplified and were polymorphic in S. arduous, S. argyreornatus, and S. faivovichi, but only one in S. alcatraz. These markers will be useful for quantifying effects of habitat fragmentation on population genetic diversity and connectivity in coastal and island populations of this threatened species group.10410531056National Science Foundation Biotic Inventory and Survey Grant [DEB-0542848]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao O BoticaroSociety of Systematic Biologists ( SSB)Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renovaveis [02027. 021071/03-04, 02010. 000492/04-81, 02010.001683/2006-22]Ministerio do Meio Ambiente [07BR000582/DF]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)National Science Foundation Biotic Inventory and Survey Grant [DEB-0542848]Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renovaveis [02027. 021071/03-04, 02010. 000492/04-81, 02010.001683/2006-22]Ministerio do Meio Ambiente [07BR000582/DF