Low-level laser therapy (LLLT) (660 nm) alters gene expression during muscle healing in rats

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Introduction: The effects of LLLT were studied during muscle regeneration through gene expression. Methods: It was evaluated 10 and 50 J/cm(2) doses during 7, 14 and 21 days post cryoinjury, through histopathological analysis and mRNA MyoD, Myogenin, Vegf and Cox-2 expression. Results: Irradiated groups presented less inflammatory process than control group after 14 and 21 days. Cox-2 levels were downregulated in all irradiated groups after 7, 14 and 21 days. On day 7, both treated groups had a downregulation of Vegf levels, and an upregulation after 14 and 21 days, mainly with 50 J/cm(2). The MyoD levels were upregulated with high dose in all periods and with low dose after 21 days. Myogenin expression was downregulated in both treated groups after 7 days, and was upregulated with 10 J/cm(2) after 21 days. Conclusion: These responses suggest that LLLT can improve the skeletal muscle regeneration through the gene expression stimulation. (C) 2013 Elsevier B.V. All rights reserved.1202935Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES