Effect Of Poly (ethylene) Glycol And Albumin On The Immobilization Of Microbial Lipase And Catalysis In Organic Media [efeito Do Polietilenoglicol E Da Albumina Na Imobilização De Lipase Microbiana E Na Catálise Em Meio Orgânico]

Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.266832838Gonçalves, A.M., Schucht, E., Matthijs, G., Aires Barros, M.R., Cabral, J.M.S., Gil, M.H., (1999) Enzyme Microb. Technol., 24, p. 60Wehtje, E., Adlercreutz, P., Mattiasson, B., (1993) Biotechnol. Bioeng., 41, p. 171Reetz, M.T., Zonta, A., Simpelkamp, J., (1996) Biotechnol. Bioeng., 49, p. 527Trintafyllou, A.O., Wehtje, E., Adlercreutz, P., Mattiasson, B., (1995) Biotechnol. Bioeng., 45, p. 406Rocha, J.M., Gil, M.H., Garcia, F.A.P., (1998) J. Biotechnol., 66, p. 61Bosley, J.A., EP pat. 424,130 1991Zanin, G.M., (1989), Tese de Doutorado, Universidade Estadual de Campinas, BrasilBaron, M., Florêncio, J.A., Zanin, G.M., Ferreira, A.G., Ennes, R., Fontana, J.D., (1996) Appl. Biochem. Biotechnol., 57-58, p. 605Soares, C.M.F., De Castro, H.F., De Moraes, F.F., Zanin, G.M., (1999) Appl. Biochem. Biotechnol., 77, p. 745Bradford, M.M.A., (1976) Anal. Biochem., 72, p. 248Box, G.E.P., Hunter, W.G., Hunter, J.S., (1978) Statistics for Experimenters: An Introduction to Design, p. 653. , Wiley & Sons Inc.: New YorkVilleneuve, P., Muderhwa, J.M., Graille, J.M., Haas, M.J., (2000) J. Mol. Catal. B: Enzym., 9, p. 11Wu, J.C., Zhang, G.F., He, Z.M., (2001) Biotechnol. Lett., 23, p. 211Harris, J.M., (1992) Em Poly (Ethylene Glycol) Chemistry: Biotechnical and Biomedical Applications, p. 228. , Plenum Press: New Yor