High-performance Liquid Chromatographic Stationary Phases Based On Poly(methyloctylsiloxane) Immobilized On Silica: Ii. Chromatographic Evaluation

This work describes the chromatographic characterization of stationary phases prepared by deposition of poly(methyloctylsiloxane) (PMOS) on silica followed by immobilization using one of several different processes: thermal treatments (120 or 220°C for 4 h), microwave irradiation (495 W for 15 min), γ radiation (dose of 80 kGy) or self-immobilization. This evaluation was based on the chromatographic parameters of several test solutes. The stationary phases immobilized at 220°C and which underwent self-immobilization were not appropriate for chromatographic use but the other immobilized phases presented chromatographic performances similar in most respects to a commercial phase (Rainin C8) while the peak characteristics of the basic probe were significantly better with these phases. © 2002 Elsevier Science B.V. All rights reserved.9481-2109119Vansant, E.F., Van Der Voort, D., Vrancken, C., (1995) Characterization and Chemical Modification of the Silica Surface, , Elsevier, New YorkPesek, J.J., Matyska, M.T., Sandoval, J.E., Williamsen, E.J., (1996) J. Liq. Chromatogr. Relat. Technol., 19, p. 2843Tani, K., Suzuki, Y., (1996) J. Liq. Chromatogr. Relat. Technol., 19, p. 3037Tani, K., Suzuki, Y., (1996) J. Chromatogr. A, 722, p. 129Sander, L.C., Wise, S.A., (1995) Anal. Chem., 67, p. 3284Sander, L.C., Pursch, M., Wise, S.A., (1999) Anal. Chem., 71, p. 4821Poole, C.F., Poole, S.K., (1991) Chromatography Today, , Elsevier, AmsterdamOhtsu, Y., Shiojima, Y., Okumura, T., Koyama, J.I., Nakamura, N., Nakata, O., Kimata, K., Tanaka, N., (1989) J. Chromatogr., 481, p. 147Engelhardt, H., Löw, H., Eberhardt, W., Maub, W., (1989) Chromatographia, 27, p. 535O'Gara, J.E., Alden, B.A., Walter, T.H., Petersen, J.S., Niederländer, C.L., Neue, U.D., (1995) Anal. Chem., 67, p. 3809Neue, U.D., Walter, T.H., Alden, B.A., Jiang, Z., Fisk, R.P., Cook, J.T., Glose, K.H., Crowley, R.J., (1999) Am. Lab., 31, p. 36Bien-Vogelsang, U., Deege, A., Figge, H., Köhler, J., Schomburg, G., (1984) Chromatographia, 19, p. 170Nyholm, L.M., Markides, K.E., (1998) J. Chromatogr. A, 813, p. 11Schomburg, G., (1991) Trends Anal. Chem., 10, p. 163Schomburg, G., Deege, A., Köhler, J., Bien-Vogelsang, U., (1983) J. Chromatogr., 282, p. 27Figge, H., Deege, A., Köhler, J., Schomburg, G., (1986) J. Chromatogr., 351, p. 393Petro, M., Berek, D., (1993) Chromatographia, 37, p. 549Mao, Y., Fung, B.M., (1997) J. Chromatogr. A, 790, p. 9Lecourtier, J., Audebert, R., Ouivovon, C., (1978) J. Liq. Chromatogr., 1, p. 367Nasal, A., Haber, P., Kaliszan, R., Forgács, E., Cserháti, T., (1996) Chromatographia, 43, p. 484Forgács, E., Cserháti, T., (1998) J. Chromatogr. A, 797, p. 33Hanson, M., Unger, K.K., Schomburg, G., (1990) J. Chromatogr., 517, p. 269Haky, J.E., Rayham, A., Dunn, B.M., (1991) J. Chromatogr., 541, p. 303Garbow, J.R., Asrar, J., Hardiman, C.J., (1993) Chem. Mater., 5, p. 869Hanggi, D.A., Marks, N.R., (1993) LC-GC, 11, p. 128Li, J., Carr, P.W., (1996) Anal. Chem., 68, p. 2857Li, J., Carr, P.W., (1996) Anal. Chim. Acta, 334, p. 239Li, J., Carr, P.W., (1997) Anal. Chem., 69, p. 2202Reeder, D.H., Li, J., Carr, P.W., Flickinger, M.C., McCormick, V., (1997) J. Chromatogr. A, 760, p. 71Li, J., Reeder, D.H., McCormick, V., Carr, P.W., (1997) J. Chromatogr. A, 791, p. 45Kurganov, A., Trüdinger, U., Isaeva, T., Unger, K.K., (1996) Chromatographia, 42, p. 217Zhao, J., Carr, P.W., (2000) Anal. Chem., 72, p. 302Mao, Y., Fung, B.M., (1997) J. Chromatogr. A, 790, p. 9Nyholm, L.M., Markides, K.E., (1998) J. Chromatogr. A, 813, p. 11Figge, H., Deege, A., Köhler, J., Schomburg, G., (1986) J. Chromatogr., 351, p. 393Schomburg, G., Köhler, J., Figge, H., Deege, A., Bien-Vogelsang, U., (1984) Chromatographia, 18, p. 265Berthod, A., (1991) J. Chromatogr., 549, p. 1Nahum, A., Horváth, C., (1981) J. Chromatogr., 203, p. 53Jardim, I.C.S.F., Collins, K.E., Anazawa, T.A., (1999) J. Chromatogr. A, 849, p. 299Silva, M.C.H., Jardim, I.C.S.F., (1998) J. Liq. Chromatogr. Relat. Technol., 21, p. 2447Collins, K.E., Bottoli, C.B.G., Bachmann, S., Vigna, C.R.M., Collins, C.H., Albert, K., Chem. Mater., , in pressTonhi, E., Bachmann, S., Albert, K., Jardim, I.C.S.F., Collins, K.E., Collins, C.H., (2002) J. Chromatogr. A, 948, p. 97Collins, K.E., Franchon, A.C., Jardim, I.C.S.F., Radovanovic, E., Gonçalves, M.C., (2000) LC-GC, 18, p. 106Bachmann, S., Melo, L.F.C., Silva, R.B., Anazawa, T.A., Jardim, I.C.S.F., Collins, K.E., Collins, C.H., Albert, K., (2001) Chem. Mater., 13, p. 1874Neue, U.D., Serowik, E., Iraneta, P., Alden, B.A., Walter, T.H., (1999) J. Chromatogr. A, 849, p. 87Galusko, S.V., (1993) Chromatographia, 36, p. 3