Preparative Chromatography Of Xylanase Using Expanded Bed Adsorption

Expanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. Experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (Streamline SP) in the presence of cells. In order to study the presence of cells, a Bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. One xylanase was purified to homogeneity in the packed bed. However, the 5% cell content hampered purification. © 2002 Published by Elsevier Science B.V.9441-2217224Bajpai, P., (1999) Biotech. Prog., 15, p. 147Shoham, Y., Schwartz, Z., Khasin, A., Gat, O., Zosim, Z., Rosemberg, E., (1992) Biodegradation, 3, p. 207Bim, M.A., Franco, T.T., (2000) J. Chromatogr. B, 743, p. 349Munk, N., (1993) Pulpzyme HC: 6 Stage ECF Bleaching, , NovonordiskSpalding, B.J., (1991) Bio/Technology, 9, p. 229Chase, H.A., (1994) Trends Biotechnol., 12, p. 296Frej, A.-K.B., Hjorth, R., Hammarström, Å., (1994) Biotechnol. Bioeng., 44, p. 922Chang, Y.-K., Chen, Y.-H., Chien, C.-H., (1998) Enzyme Microbial Technol., 23, p. 204Raymond, F., Rolland, D., Gauthier, M., Jolivet, M., (1998) J. Chromatogr. B, 706, p. 113Hamilton, G.E., Luechau, F., Burton, S.C., Lyddiatt, A., (2000) J. Biotechnol., 79, p. 103(1996) Handbook of Expanded Bed Adsorption - Principles and Methods, , Amersham Pharmacia Biotech, Uppsala, SwedenDuarte, M.C.T., Portugal, E.P., Ponezi, A.N., Bim, M.A., Tagliari, C.V., Franco, T.T., (1999) Bioresour. Technol., 62, p. 49Bailey, M.J., Biely, P., Poutanen, K., (1992) J. Biotech., 23, p. 257Miller, G.L., (1959) Anal. Chem., 31, p. 426Sedmak, J.J., Grossberg, S.E., (1977) Anal. Biochem., 79, p. 544Laemmli, U.K., (1970) Nature, 227, p. 680Chang, Y.K., Chase, H.A., (1996) Biotechnol. Bioeng., 49, p. 204Chang, Y.K., Chase, H.A., (1996) Biotechnol. Bioeng., 49, p. 512Batt, B.C., Yabannavar, V.M., Singh, V., (1995) Bioseparation, 5, p. 41Goto, M., Imamura, T., Hirose, T., (1995) J. Chromatogr. A, 690, p. 1Santos, E.S., Guirardello, R., Franco, T.T., (2000) Ion Exchange at the Millenium (Proceedings of IEX 2000), , J.A. Greig (Ed.), SCI, LondonFernández-Lahore, H.M., Kleef, R., Kula, M.-R., Thömmes, J., (1999) Biotechnol. Bioeng., 64, p. 48