Induction Of Secondary Metabolites In Plantlets Of Hypericum Brasiliense Choisy In Vitro [indução De Metabólitos Secundários Em Plântulas De Hypericum Brasiliense Choisy Crescendo In Vitro]

The production of rutin, quercetin, 1,5-dihydroxyxanthone and betulinic acid was investigated in plantlets of H. brasieliense in vitro, and exposed to salycilic acid, poliethylene glycol, NaCl, 24-epibrassinolide, benzothiadiazole (BION), methyljasmonate and increased concentrations of boron and nitrogen in the liquid culture medium. Evaluations of the contents were carried out after 5 and 10 days of treatments. The highest increase was observed in quercetin in the salycilic acid and B treatments after 5 days of exposure, and in 24-epibrassinolide and BION after 10 days.392267272Abreu, I.N., Porto, A.L.M., Marsaioli, A.J., Mazzafera, P., Distribution of bioactive substances from Hypericum brasiliense during plant growth (2004) Plant Science, 167, pp. 949-954Abreu, I.N., Mazzafera, P., Effects of water and temperature stress of activity constituent Hypericum brasiliense Choisy (2005) Plant Physiology and Biochemistry, 43, pp. 241-248Abreu, I.N., (2002) Isolamento e produção de substâncias de interesse farmacológico de Hypericum brasiliense Choisy, , Tese de doutorado, Universidade Estadual de Campinas, Campinas, São PauloAvancini, G., Abreu, I.N., Saldaña, M.D.A., Mohamed, R.S., Mazzafera, P., Induction of pilocarpine formation in jaborandi leaves by salicylic acid and methyljasmonate (2003) Phytochemistry, 63, pp. 171-175Barber, G.A., Behrman, E.J., The synthesis and characterization of uridine 5'-(beta-L-rhamnopyranosyl diphosphate) and it role in the enzymatic-synthesis of rutin (1991) Archives of Biochemistry and Biophysics, 288, pp. 239-242Beerhues, L., Berger, U., Differential accumulation of xanthone in methyl jasmonate and yeast extract treated cell cultures of C. erythraea and C. littorale (1995) Planta, 197, pp. 608-612Brachet, J., Cosson, L., Changes in the total alkaloid content of Datura inoxia Mill. subjected to salt stress (1983) Journal of Experimental Botany, 37, pp. 650-656Cardoso, M.A., Oliveira, D.E., Tissue culture of Hypericum brasiliense Choisy: Shoot multiplication and callus induction (1996) Plant Cell Tissue and Organ Culture, 44, pp. 91-94Choe, S., Brassinosteroid biosynthesis and inactivation (2006) Physiologia Plantarum, 126, pp. 539-548Conceição, L.R.F., Ferreres, F., Tavares, R.M., Induction of phenolic compounds in Hypericum perforatum L. cells by Colletotrichum gloesporioides elicitation (2006) Phytochemistry, 67, pp. 149-155Croteau, R., Kutchan, T.M., Lewis, N.G., Natural products (Secondary metabolites) (2000) Biochemistry and Molecular Biology of Plants, pp. 1250-1318. , In: Buchanan, B.Gruissem, W.Jones, R. (Ed.)., American Society of Plant Physiologists, RockvilleDixon, R.A., Paiva, N., Stress-induced phenylpropanoid metabolism (1995) Plant Cell, 7, pp. 1085-1097Fujita, M., Fujita, Y., Noutoshi, Y., Takahashi, F., Narusaka, Y., Yamaguchi-Shinozaki, K., Shinozaki, K., Crosstalk between abiotic and biotic stress responses: A current view from the points of convergence in the stress signaling networks (2006) Current Opinion in Plant Biology, 9, pp. 436-442Hammerschmidt, R., Induced disease resistance: How do induced plants stop pathogens? (1999) Physiological and Molecular Plant Pathology, 55, pp. 77-84Kirakosyan, A., Hayashi, H., Inoue, K., Charchoglyan, A., Vardapetyan, H., Stimulation of the production of hypericins by mannan in Hypericum perforatum shoot cultures (2000) Phytochemistry, 53, pp. 345-348Mazzafera, P., Mineral nutrition and caffeine content in coffee leaves (1999) Bragantia, 58, pp. 387-391McConn, M., Creelman, R.A., Bell, E., Mullet, J.E., Browse, J., Jasmonate is essential for insect defense in Arabidopsis (1997) Proceedings of the National Academy of Science USA, 94, pp. 5473-5477Nuñez, M., Mazzafera, P., Mazorra, L.M., Siqueira, W.J., Zullo, M.A.T., Influence of a brassinosteroid analogue on antioxidant enzymes in rice growing in culture medium with NaCl (2003) Biologia Plantarum, 47, pp. 67-70Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture (1962) Plant Physiology, 15, pp. 473-497Nugroho, L.H., Verberne, M.C., Verpoorte, R., Activities of enzymes involved in the phenylpropanoid pathway in constitutively salicylic acid-producing tobacco plants (2002) Plant Physiology Biochemistry, 40, pp. 755-760Oliveira, B.H., Santos Cid., A.M., Espíndola, A.P.D.M., Determination of the triterpenoid, betulinic acid, in Doliocarpus schottianus by HPLC (2002) Phytochemical Analysis, 13, pp. 95-98Pasqua, G., Avato, P., Monacelli, B., Santamaría, A.R., Argentieri, M.P., Metabolites in cell suspension cultures, calli, and in vitro regenerated organs of Hypericum perforatum cv (2003) Topas. Plant Science, 165, pp. 977-982Pozo, M.J., Loon, L.C.V., Pieterse, C.M.J., Signals in plant-microbe interactions (2004) Journal of Plant Growth Regulation, 23, pp. 211-222Rocha, L., Marston, A., Kaplan, M., Stoecklievans, H., Thull, U., Testa, B., Hostettmann, K., An antifungal γ-pyrone and xanthones with monoamine oxidase inhibitory activity from Hypericum brasiliense (1994) Phytochemistry, 36, pp. 1381-1385Rocha, L., Marston, A., Potterat, O., Kaplan, M., Evans, H., Hostettmann, K., Antibacterial phloroglucinols and flavonoid from Hypericum brasiliense (1995) Phytochemistry, 40, pp. 1447-1452Rocha, L., Marston, A., Potterat, O., Kaplan, M., Hostettmann, K., More phoroglucinols from Hypericum brasiliense (1996) Phytochemistry, 42, pp. 185-188Saenz, L., Santamaría, J.M., Villanueva, M.A., Loyola-Vargas, V.M., Oropeza, C., Changes in the alkaloid content of plants of Catharanthus roseus L. (Don). as a result of water stress and treatment with abscisic acid (1993) Journal of Plant Physiology, 142, pp. 244-247Sirvent, T., Gibson, D., Induction of hypericins and hyperforin in Hypericum perforatum L. in response to biotic and chemical elicitors (2002) Physiological and Molecular Plant Pathology, 60, pp. 311-320Suzuki, O., Katsumata, Y., Oya, M., Inhibition of monoamine oxidase by hypericin (1984) Planta Medica, 50, pp. 272-274Thomma, B.P.H.J., Eggermont, K., Broekaert, W.F., Cammue, B.P.A., Disease development of several fungi on Arabdopsis can be reduced by treatment with methyl jasmonate (2000) Plant Physiology Biochemistry, 38, pp. 421-427Tramontano, W.A., Jouve, D., Trigonelline accumulation in salt-stressed legumes and the role of other osmoregulators as cell cycle control agents (1997) Phytochemistry, 44, pp. 1037-1040Vermerris, W., Nicholson, R., Phenolic compound biochemistry (2006), p. 276. , Springer, DordrechtVerpoorte, R., van der Heijden, R., Ten Hoopen, H.J.G., Memelink, J., Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals (1999) Biotechnology Letters, 21, pp. 467-479Walker, T.S., Bais, H.P., Vivanco, J.M., (2002) Phytochemistry, 60 (3), pp. 289-293Yu, K.W., Gao, W.Y., Hahn, E.J., Paek, K.Y., Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng CA Meyer (2002) Biochemical Engineering Journal, 11, pp. 211-215Zenk, M.H., El Shagi, H., Arens, H., Stockigt, J., Weiler, E.W., Deus, B., (1977) Plant Tissue culture and its bio-technological application, pp. 27-47. , In: Bars, W.Reinhard, E.Zenk, M.H. (Eds), Springer-Verlag, Berlin-HeidelbergZobayed, S.M.A., Murch, S.J., Rupasinghe, H.P.V., Saxena, P.K., In vitro production and chemical characterization of St. John's wort (Hypericum perforatum L. cv 'New Stem') (2004) Plant Science, 166, pp. 333-340Zullo, M.A.T., Adam, G., Brassinosteroid phytohormones - structure, bioactivity and applications (2002) Brazilian Journal of Plant Physiology, 14, pp. 143-18