Electrochemical Biosensor-based Devices For Continuous Phenols Monitoring In Environmental Matrices

A flow system method for continuous determination of phenolic compounds in environmental matrices was employed using a dialysis membrane sampler and laccase- and tyrosinase-based biosensors as detector. The biosensors response to different phenolic compounds was investigated. The laccase-based biosensor showed high sensitivity to guaiacol and chloroguaiacol, while the tyrosinase-based biosensor was more sensitive to phenol and chlorophenol. Both of the biosensors presented highly selective measurements of micromolar concentration of these compounds. Detection limits around 1.1 x 10-7 mol L-1 for guaiacol and 1.9 x 10-7 mol L-1 for chloroguaiacol could be estimated for the laccase-based biosensor. Using the tyrosinase-based biosensor detection limits of 1.5 x 10-7 mol L-1 for phenol and 9.0 x 10-8 mol L-1 for chlorophenol were observed. The proposed flow method presented a linear response range between 1.0 μmol L-1 and 100.0 μmol L-1, in the optimized operational conditions (laccase-based biosensor: pH 5.0 and 0 mV vs Ag/AgCl as working potential; tyrosinase-based biosensor: pH 5.5 and 50 mV vs Ag/AgCl as working potential). Real paper mill effluent sample was analyzed by this system and by the reference colorimetric method and the results were discussed.134456462Ortega, F., Domínguez, E., Burestedt, E., Emneus, J., Gorton, L., Marko-Varga, G., (1994) J. Chromatogr., A, 675, p. 65Rogers, K.M., Becker, J.Y., Cembrana, J., Chough, S.H., (2001) Talanta, 54, p. 1059Russell, I.M., Burton, S.G., (1999) Anal. Chim. Acta, 389, p. 161Rossato, S.S., Freire, R.S., Durán, N., Kubota, L.T., (2001) Quim. Nova, 24, p. 77Canofeni, S., DiSario, S., Mela, J., Pilloton, R., (1994) Anal. Lett., 27, p. 1659Nistor, C., Emneus, J., Gorton, L., Ciucu, A., (1999) Anal. Chim. Acta, 387, p. 309Gundersen, J.L., Macintyre, W.G., Hall, R.C., (1997) Environ. Sci. Technol., 31, p. 188Wang, J., (1997) Trends Anal. Chem., 16, p. 84Wang, J., Lu, J., Ly, S.Y., Tian, B., Adeniyi, W.K., Armendariz, R.A., (2000) Anal. Chem., 72, p. 2659Wang, J., Chen, Q., (1995) Anal. Chim. Acta, 312, p. 39Cosnier, S., Fombon, J.J., Labbe, P., Limosin, D., (1999) Sens. Actuators, B, 59, p. 134Yaropolov, A.I., Kharybin, A.N., Emnéus, J., Marko-Varga, G., Gorton, L., (1995) Anal. Chim. Acta, 308, p. 137Freire, R.S., Durán, N., Kubota, L.T., (2001) Talanta, 54, p. 681Freire, R.S., Thongngamdee, S., Durán, N., Wang, J., Kubota, L.T., (2001) Analyst, 127, p. 258(1989) Standard Methods for Examination of Water and Waste-water, 17th ed., , Amer. Publ. Health Ass. N0 5550 New YorkFreire, R.S., Kubota, L.T., Durán, N., (2001) Environ. Technol., 22, p. 897Burestedt, E., Narvaez, A., Ruzgas, T., Gorton, L., Emneus, J., Dominguez, E., Marko-Varga, G., (1996) Anal. Chem., 68, p. 1605Rogers, K.R., Becker, J.Y., Cembrano, J., (2000) Electrochim. Acta, 45, p. 4373Marko-Varga, G., Emneus, J., Gorton, L., Ruzgas, T., (1995) Trends Anal. Chem., 14, p. 319Freire, R.S., Duran, N., Kubota, L.T., (2002) Anal. Chim. Acta, , in pres