Cyclodextrin Glycosyltransferase Production By The Bacillus Sp., Subgroup Alcalophilus Using A Central Composite Design

Cyclodextrin glycosyltransferase (CGTase) activity was produced by the Bacillus sp., subgroup alcalophilus in a culture medium containing cassava starch. A central composite design and response surface methodology were used to study the influence of carbon source (cassava starch), nitrogen sources (yeast extract and tryptone) and sodium carbonate in the production medium. Assays were performed in 300 mL Erlenmeyer flasks containing 100 mL of production medium maintained in a shaker at 150 rpm at 35±1°C for 72 h of fermentation. The independent variables [0.75% cassava starch, nitrogen sources (0.375% yeast extract and 0.375% tryptone) and 1% Na2CO3] produced an enzyme activity of 96.07 U mL-1.© Academic Journals Inc.411450459Abdel-Shafi, A.A., Ai-Shihry, S.S., Fluorescence enhancement of 1-napthol-5-sulfonate by forming inclusion complex with β-cyclodextrin in aqueous solution (2009) Spectrochim Acta A, 72, pp. 533-537Adriana, M.R., Susana, A.F., Norberto, K., Clara, B.N., Optimisation of batch culture conditions for cyclodextrin glucanotransferase from Bacillus circulans DF 9R (2002) Microl. Cell Fact, 1, pp. 1-9Aime, S., Gianolio, E., Arena, F., Barge, A., Martina, K., Heropoulosc, G., Cravotto, G., New cyclodextrin dimers and trimers capable of forming supramolecular adducts with shape-specific ligands (2009) Org. Biomol. Chem, 7, pp. 370-379Biwer, A., Antranikian, G., Heinzle, E., Enzymatic production of cyclodextrins (2002) Applied Microbiol. Biotechnol, 59, pp. 609-617Cho, S.H., Kim, S.Y., Lee, S.I., Lee, Y.M., Hydroxypropyl-β-cyclodextrin inclusion complexes for transdermal delivery: Preparation, inclusion properties, stability and release behavior (2001) J. Ind. Eng. Chem, 12, pp. 50-59Freitas, T.L., Monti, R., Contiero, J., Production of CGTase by a Bacillus alcalofilico CGII Strain isolated from wastewater of manioc flour industry (2004) Braz. J. Microb, 35, pp. 225-260Gornas, P., Neunert, G., Baczynski, K., Polewski, K., Beta-cyclodextrin complexes with chlorogenic and caffeic acids from coffee brew: Spectroscopic, thermodynamic and molecular modelling study (2009) Food Chem, 114, pp. 190-196Haiyee, Z.A., Saim, O., Said, M., Illias, R.M., Mustapha, W.A.W., Hassan, O., Characterization of cyclodextrin complexes with turmeric oleoresin (2009) Food Chem, 114, pp. 459-465Higuti, I.H., Grande, S.W., Sacco, R., Nascimento, A.J., Isolation of alkalophilic CGTase-producing bacteria and characterization of cyclodextrin-glycosyltransferase (2003) Braz. Archives Biol. Technol, 46, pp. 183-186Jukes, T.H., Cantor, C.R., Evolution of Protein Molecules (1969) Mammalian Protein Metabolism, pp. 21-132. , Munro, H.N. (Ed.). Academic Press, New York, ppKhairizal, M.M., Rosli, M.I., Roshanida, A.R., Noor, A.A.R., Nik, A.N.M., Osman, H., Suraini, A.A., Kamarulzaman, K., Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1: Media optimization using experimental design (2004) Enzyme Microb. Technol, 35, pp. 467-473Makela, M.J., Korpela, T.K., Puisto, J., Laakso, S.V., Nonchromatographic cyclodextrin assays: Evaluation of sensitivity, specificity and conversion mixture applications (1988) J. Agric. Food Chem, 36, pp. 83-88Moraes, C.M., Abrami, P., Filho, M.M.G.N.A., Fernandes, S.A., Paula, E., Fraceto, L.F., Preparacao e caracterizacao físico-quimica de complexos de inclusão entre anaestésicos locais e hidroxil-β-ciclodextrina (2007) Quim. Nova, 30, pp. 777-784Nakamura, N., Horikoshi, K., Characterization and some cultural conditions of a cyclodextrin glycosyltransferase-producing alkalophilic Bacillus sp (1976) Agric. Biol. Chem, 40, pp. 753-757Provenzi, G., Falcão, L.D., Fett, R., Luiz, M.T.B., Estabilidade de antocianinas de uvas cabernet sauvignon com β- e γ-ciclodextrinas (2006) Braz. J. Food Technol, 9, pp. 165-170Qi, Q., She, X., Endo, T., Zimmermann, W., Effect of the reaction temperature on the transglycosylation reactions catalyzed by the cyclodextrin glucanotransferase from Bacillus macerans for the synthesis of large-ring cyclodextrins (2004) Tetrahedron, 60, pp. 799-806Ribeiro, A.O., Serra, O.A., Spectroscopic study of Tb3+(b-diketonate)3: α-cyclodextrin inclusion compounds in aqueous solution (2007) J. Braz. Chem. Soc, 18, pp. 273-278Saitou, N., Nei, M., The neighbor-joining method: A new method for reconstructing phylogenetic trees (1987) Mol. Biol. Evol, 4, pp. 406-425Seon, K.H., Ahn, J., Kwak, H.S., The accelerated ripening of cholesterol-reduced cheddar cheese by crosslinked β-cyclodextrin (2009) J. Dairy Sci, 92, pp. 49-57Szente, L., Szejtli, J., Cyclodextrins as food ingredients. Trends Food Sci (2004) Trends Food Sci. Tech, 15, pp. 137-142Xu, D., Côté, J.C., Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3′ end 16S rDNA and 5 end 16S-23S ITS nucleotide sequences (2003) Int. J. Syst. Evol. Micr, 53, pp. 695-704Zain, W.S.W.M., Illias, R.M., Salleh, M.M., Hassan, O., Rahman, R.A., Hamid, A.A., Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1: Optimization of carbon and nitrogen concentration in the feed medium using central composite design (2007) Biochem. Eng. J, 33, pp. 26-3