Blood Protein Adsorption On Sulfonated Chitosan And κ-carrageenan Films

Many strategies have been reported to improve compatibility of biopolymers using chemical and physical modifications. One possibility is the introduction of sulfonate groups (R-SO3 -) in the chitosan chain. Another biopolymer with similar characteristics to those of heparin is κ-carrageenan. This study proposed to investigate the application of these two polymers, based on their potential for globular protein adsorption (BSA and fibrinogen). Polymeric films of chitosan and κ-carrageenan were prepared; all films were characterized by elemental analyses, FTIR, XPS and SEM. Characterization techniques showed that the chitosan chain was modified and confirmed the existence of sulfonate groups, as well as in the κ-carrageenan chain, indicating surfaces with similar chemical properties to those of heparin. The effect of charge density was observed for each adsorption condition (BSA at pH 5.0 and 7.4). A more pronounced adsorption rate was observed at pH 5.0 than at pH 7.4 and equilibrium adsorption was achieved, in both cases, after approximately 20min. The equilibrium data indicate a lower adsorption rate for the sulfonated chitosan film, in comparison to the other films. These results confirm the potential of modified chitosan for use in applications in which globular protein adsorption should be avoided. © 2013 Elsevier B.V.111719725(1986) Chitin in Nature and Technology, , Plenum Press, New York, R. Muzzarelli, C. Jeuniaux, G.W. Gooday (Eds.)Li, Q., Dunn, E.T., Grandmaison, E.W., Goosen, M.F.A., (1992) J. Bioact. Compat. Polym., 7, p. 370Baier, R.E., (1978) Artif. Organs, 2, p. 422Salzman, E.W., Lindon, J., Brier, D., Merril, E.W., Surface-induced platelet adhesion, aggregation and release (1977) Ann. N. Y. Acad. Sci., 283, p. 114Anderson, J., Kottke-Merchant, K., (1985) CRC Crit. Rev. Biocomp., 1, p. 111Hoffman, A., Blood-biomaterial interactions: an overview (1982) Biomaterials: Interfacial Phenomena and Applications, p. 3. , American Chemical Society, Washington, DC, S.L. Coppper, N.A. Peppas (Eds.)Amiji, M., (1998) Colloids Surf. B, 10, p. 263Hu, J., Li, S., Liu, B., (2005) Biochem. Eng. J., 23, p. 259Benesh, J., Tengvall, P., (2002) Biomaterials, 23, p. 2561Drozd, M.M., Sher, A.I., Makarov, V.A., Galbraikh, L.S., Vikhoreva, G.A., Gorbachiova, I.N., (2001) Thromb. Res., 102, p. 445Muzzarelli, R.A.A., (1992) Carbohydr. Polym., 19, p. 231Amiji, M.M., (1997) Carbohydr. Polym., 32, p. 193Zablackis, E., Santos, G.A., (1986) Bot. Mar., 29, p. 319Hugerth, A., Caram-Lelham, N., Sundelöf, L.O., (1997) Carbohydr. Polym., 34, p. 149Montero, P., Perez-Mateos, M., (2002) Food Hydrocolloids, 16, p. 375Beppu, M.M., Santana, C.C., (2003) Mater. Sci. Eng., 23 C, p. 651Hoven, V.P., Tangpasuthadol, V., Angkitpaiboon, Y., Vallapa, N., Kiatkamjornwong, S., (2007) Carbohydr. Polym., 68, p. 44Arof, A.K., Osman, Z., (2003) Electrochim. Acta, 48, p. 993Wang, X., Du, Y., Liu, H., (2004) Carbohydr. Polym., 56, p. 21Tapia, C., Escobar, Z., Costa, E., Sapag-Hagar, J., Valenzuela, F., Basualto, C., Gai, M., Yazdani-Pedram, M., (2004) Eur. J. Pharm. Biopharm., 57, p. 65Volery, P., Besson, R., Schaffer-Lequart, C., (2004) J. Agric. Food Chem., 52, p. 7463Abad, L.V., Relleve, L.S., Aranilla, C.T., Rosa, A.M.D., Proprieties of radiation synthesized PVP-kappa carrageenan hydrogel blends (2003) Rad. Phys. Chem., 68, p. 901Vikhoreva, G., Bannikova, B., Stolbushkina, P., Panov, A., Drozd, N., Makarov, V., Varlamov, V., Gal'braikh, L., (2005) Carbohydr. Polym., 62, p. 327Vogler, E.A., (2012) Biomaterials, 33, pp. 1201-123