Electrochemical Oxidation, Adsorption And Quantification Of 1,2-benzopyrone Employing A Glassy Carbon Electrode

The electrooxidation of 1,2-benzopyrone (BP) was assessed via linear voltammetry and electrochemical impedance spectroscopy on a glassy carbon electrode in dibasic potassium phosphate buffer. The oxidation process for BP requires one electron and forms a ketone. This oxidation product was adsorbed by the electrode surface to form a film that blocks active sites and increases in thickness over consecutive measurements. The oxidation conditions were optimized using factorial design and Doehlert matrices. This electrochemical method was compared to high performance liquid chromatography (HPLC), which has a detection limit of 26.4 μmol L-1 for BP. The voltammetric results were statistically similar to those from HPLC; however, the method was faster, simpler, more easily acquired, more sensitive, and required less organic solvent. © 2014 Sociedade Brasileira de Quimica.253469477Lake, B.G., (1999) Food Chem. Toxicol, 37, p. 423Razavi, S.M.N., Nazemiyed, H., Hajiboland, R., Kumarasamy, Y., Delazar, A., Nahar, L., Sarker, S.D., (2008) Braz. J. Pharmacogn, 18, p. 1Thati, B., Noble, A., Rowan, R., Creaven, B.S., Walsh, M., McCann, M., Egan, D., Kavanagh, K., (2007) Toxicol. in Vitro, 21, p. 801Lino, C.S., Taveira, M.L., Viana, G.S.B., Matos, F.J.A., (1997) Phytother. Res, 11, p. 211Finn, G.J., Creaven, B.S., Egan, D.A., (2004) Cancer Lett, 205, p. 69Capra, J.C., Cunha, M.P., Machado, D.G., Zomkowski, A.D.E., Mendes, B.G., Santos, A.R.S., Pizzolatti, M.G., Rodrigues, A.L.S., (2010) Eur. J. Pharmacol, 643, p. 232Vergel, N.E., López, J.L., Orallo, F., Viña, D., Buitrago, D.M., Del Olmo, E., Mico, J.A., Guerrero, M.F., (2010) Life Sci, 86, p. 819Pereira, E.C., Lucetti, D.L., Barbosa-Filho, J.M., De Brito, E.M., Monteiro, V.S., Patrocínio, M.C.A., De Moura, R.R., Vasconcelos, S.M.M., (2009) Neurosci. Lett, 454, p. 139Ariza, S.Y., Rueda, D.C., Rincón, V.J., Linares, E.L., Guerrero, M.F., (2007) Vitae, 14, p. 51Celeghini, R.M.S., Vilegas, J.H.Y., Lanças, F.M., (2001) J. Braz. Chem. Soc, 12, p. 706Osório, A.C., Martins, J.L.S., (2004) Rev. Bras. Cienc. Farm, 40, p. 481Vilegas, J.H.Y., De Marchi, E., Lancas, F.M., (1997) Phytochem. Anal, 8, p. 266Šeruga, M., Novak, I., Jakobek, L., (2011) Food Chem, 124, p. 1208Tarley, C.R.T., Silveira, G., Santos, W.N.L., Matos, G.D., Silva, E.G.P., Bezerra, M.A., Miró, M., Ferreira, S.L.C., (2009) Microchem. J, 92, p. 58Dempsey, E., O'Sullivan, C., Smyth, M.R., Egan, D., O'Kennedys, R., Wang, J., (1993) J. Pharmaceut. Biomed, 6, p. 443Wang, L.-H., Liu, H.-H., (2009) Molecules, 14, p. 3538Ferreira, S.L.C., Queiroz, A.S., Fernandes, M.S., Santos, H.C., (2002) Spectrochim. Acta B, 57, p. 1939Harle, A.J., Lyons, L.E., (1950) J. Chem. Soc, 325, p. 1575Bard, A.J., Faulkner, L.R., (2001) Electrochemical Methods: Fundamentals and Applications, , 5th ed Wiley: New York, USASerrano, K., Taxil, P., (1999) J. Appl. Electrochem, 29, p. 497Cougnon, C., (2006) Electrochim. Acta, 51, p. 4142Michalkiewicz, S., (2013) J. Appl. Electrochem, 43, p. 85Sotomayor, M.D.P.T., Sigoli, A., Lanza, M.R.V., Tanaka, A.A., Kubota, L.T., (2008) J. Brazil Chem. Soc, 19, p. 734Barba, F., Elinson, M.N., Escudero, J., Feducovich, S.K., (1997) Tetrahedron, 53, p. 4427Rarba, F., Guirado, A., Barba, I., Lopez, M., (1982) Tetrahedron Lett, 23, p. 463Sílvio, L.P.D., Fujiwara, S.T., Gushikem, Y., Bruns, R.E., (2002) J. Electroanalytical Chem, 531, p. 141Thomas, F.G., (1993) J. Electroanal. Chem, 349, p. 8