Microsatellite Markers For The Endangered Orchids Cattleya Labiata Lindl. And C. Warneri T. Moore (orchidaceae)

In this study, we developed a new set of microsatellite markers for endangered Cattleya labiata and Cattleya warneri. Also the first microsatellite loci for Cattleya, these markers can be useful for studies in the conservation genetics of these species. Twenty-one new microsatellite primers pairs were characterized by cloning an enriched library, and two libraries were obtained by cloning inter-simple sequence repeats. Thirteen polymorphic primers were found, and the remaining eight were monomorphic for both species. Our results indicate that these new microsatellite markers have the potential to be used as a tool for studying the genetic diversity of C. labiata and C. warneri and for future studies focusing mating systems and the detection of hybrids. © 2013 Springer Science+Business Media Dordrecht.53791794Barros, F., Vinhos, F., Rodrigues, V.T., Berberena, F.F.V.A., Fraga, C.N., Orchidaceae (2010) Catálogo De Plantas E Fungos Do Brasil, 2, pp. 1344-1426. , R. Forzza (Ed.), Rio de Janeiro: Andrea Jakobsson EstúdioDoyle, J.J., Doyle, J.L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue (1987) Phytochem Bull, 19, pp. 11-15Kemp, S., (2002) PIC Calculator Extra, , http://www.genomics.liv.ac.uk/animal/pic.html, Accessed 1 July 2010Leach, R., (2000) Perfect Microsatellite Repeat Finder, , http://sgdp.iop.kcl.ac.uk/nikammar, Accessed 1 July 2010Mudunuri, S., Nagarajaram, H., IMEx: imperfect microsatellite extractor (2007) Bioinformatics, 23, pp. 1181-1187Peakall, R., Smouse, P.E., GenAlEx 6.5: genetic analysis in excel. Population genetic software for teaching and research-an update (2012) Bioinformatics, 19, pp. 2537-2539Provan, J., Wilson, P.J., Development of microsatellites for the peat moss Sphagnum capillifolium using ISSR cloning (2007) Mol Ecol Notes, 7, pp. 254-256Rozen, S., Skaletsky, H., Primer3 on the WWW for general users and for biologist programmers (2000) Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365-386. , S. Krawetz and S. Misener (Eds.), Totowa, New Jersey, USA: Humana Pressvan den Berg, C., New combinations in the genus Cattleya Lindl. (Orchidaceae) (2008) Neodiversity, 3, pp. 3-12van den Berg, C., Hinggins, W.E., Dressler, R.L., Whitten, W.M., Soto-Arenass, M.A., Chase, M.W., A phylogenetic study of Laeliinae (Orchidaceae) based on combined nuclear and plastid DNA sequences (2009) Ann Bot, 104, pp. 417-43