In Vivo Toxicity Of Enoxaparin Encapsulated In Mucoadhesive Nanoparticles: Topical Application In A Wound Healing Model

Wound healing comprises four distinct phases and involves many cell events and biologic markers. The use of nanoparticles for topical application has gaining attention due to its deeper penetration in the skin and the retention capacity of the drug in the site of application. In this study the effect and toxicity of mucoadhesive polymeric nanoparticles loaded with enoxaparin was evaluated in in vivo model of skin ulcer. Our results showed an interesting formulation based on mucoadhesive nanoparticles with enoxaparin that improved wound healing without cytotoxicity in vitro in all endpoint evaluated. Then, this semi-solid formulation is a promising option for skin ulcer treatment. © IOP Publishing Ltd 2013.4291Lazarus, G.S., (1994) Arch. Dermatol., 130 (4), p. 489. , 10.1001/archderm.1994.01690040093015 0003-987XDiegelmann, R.F., Evans, M., (2004) Frontiers Biosci., 9 (1-3), p. 283. , 10.2741/1184 1093-9946MacKay, D., Miller, A.L., (2003) Alternative Med. Rev., 8, p. 359Mishra, M., (2008) Dig. J. Nanomater. Bios., 3, p. 49Barreto, M.G.P., (2010) Rev. Assoc. Méd. Bras., 56 (1), p. 51. , 10.1590/S0104-42302010000100016 0104-4230Ravikumar, T., (2006) Ann. Burn Fire Disasters., 19, p. 123Guterres, S.S., (2007) Drug Target Insights, 2, p. 147Silva, H.S.R., (2006) Quím. Nova., 29 (4), p. 776. , 10.1590/S0100-40422006000400026 0100-4042Okamoto, Y., (1992) Elsevier, p. 70. , 0922-3444Eidi, H., (2010) Int. J. Pharm., 396 (1-2), p. 156. , 10.1016/j.ijpharm.2010.06.006 0378-5173Bryan, N., (2012) Eur.Cells Mater., 24, p. 249Meng, F.T., (2003) J.Control. Release., 91 (3), p. 407. , 10.1016/S0168-3659(03)00273-6 0168-3659Lam, L.H., (1976) Biochem Biophys Res Commun., 69 (2), p. 570. , 10.1016/0006-291X(76)90558-1 0006-291XAebi, H., (1984) Methods Enzymol., 105, p. 121. , 10.1016/S0076-6879(84)05016-3 0076-6879Yagi, K., (1987) Chem. Phys Lip., 45 (2-4), p. 337. , 10.1016/0009-3084(87)90071-5 0009-3084Green, L.C., (1982) Analytical Biochemestry, 126 (1), p. 131. , 10.1016/0003-2697(82)90118-X 0003-2697Melo, M.G.D., (2012) Scientia Plena., 8, p.