Separation Of Bovine Serum Albumin And Cytochrome C Using Gas-liquid Interface Adsorption

Adsorptive bubble separation technique is especially advantageous in treating mixtures of dilute liquid solutions of compounds that have different degrees of hydrophobicity like protein molecules. In this work we demonstrate this separation technique by studying the effects of important variables such as the pH solution, and the superficial gas velocity (Vgas) on the performance of a bubble and foam fractionation column for separating binary mixtures of bovine serum albumin (BSA) and cytochrome C. The results showed that the pH plays a very important role in this process. Also, the superficial gas velocity was proven to be a very important factor for controlling the concentration of the top product due to its effects on the drainage phenomenon.2828184Santana, C.C., Adsorptive bubble separation processes as a means of reducing surface-active contaminants in industrial wastewaters (1994) Revista Brasileira de Engenharia, 5 (OCTOBER). , in PortugeseUraizee, F., Narsimhan, G., (1990) Enz.Microb.Technol., 12, pp. 232-233Uraizee, F., Narsfmhan, G., (1995) Sep.Sci.Technol., 30 (6), pp. 847-881Bhattacharya, P., Ghosal, S.K., Sen, K., (1994) Sep.Sci.Technol., 29 (7), pp. 855-865Desouza, A.H.G., Tanner, R.D., Effler, W.T., (1997) Appl.Biochem.Biotech., 28-29, pp. 655-666Lemlich, R., (1972) Adsorptive Bubble Separation Techniques, pp. 33-51. , Academic Press, New York, Ch.3, Ch.7, pp. 133-143Grieves, R.B., (1982) Treatise on Anal.Chem., 5 (9), pp. 371-448Thomas, A., Winkler, M.A., (1977) Topics in Enzyme and Fermentation Biotechnology, pp. 43-71. , Ed. A. Wiseman, Ch.3De Lucena, S.L., Miranda, E.A., Santana, C.C., (1996) Appl.Biochem.Biotech., (57-58), pp. 57-65Ostermaier, K., Dobias, B., (1985) Coll.Surf., (14), pp. 199-20