Bluetongue Virus: Production And Study Of Viral Antigen For Serological Diagnosis

A soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 103 and 25 × 103 NMWP, showed complete identity to standard antigens when compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, revealing that the main protein component responsible for the AGID reaction has a molecular weight of about 60 kDa corresponding probably to the NS1 protein. © 1993.4402/03/15281286Adams, Gogolewski, Barbet, Cheevers, Identification of caprine arthritisencephalitis retrovirus proteins in immunodiffusion precipitin line (1985) J. Gen. Virol., 66, pp. 1139-1143Campbell, Grubman, Current knowledge on the biochemistry and immunology of bluetongue (1985) Prog. Vet. Microbiol. Immunol., 1, pp. 58-79Eaton, Hyatt, White, Localization of the nonstructural protein NS1 in bluetongue virus-infected cells and its presence in virus particles (1988) Virology, 163, pp. 527-537Hubschle, Yang, Immunodiffusion studies with bluetongue virus using an isolated core protein (1983) Proc. Am. Assoc. Vet. Lab. Diagn., 26, pp. 725-730Huismans, Protein synthesis in bluetongue virus-infected cells (1979) Virology, 92, pp. 385-396Huismans, Cloete, A comparison of different cloned bluetongue virus genome segments as probes for the detection of virus-specified RNA (1987) Virology, 158, pp. 373-380Huismans, Els, Characterization of the tubules associated with the replication of three different orbiviruses (1979) Virology, 92, pp. 397-406Huismans, Bremer, Barber, The nucleic acid and proteins of epizootic haemorrhagic disease virus (1979) J. Vet. Res., 46, pp. 51-58Jochim, Chow, Immunodiffusion of bluetongue virus (1969) Am. J. Vet. Res., 30, pp. 33-41Jochim, Improvement of the AGP test for bluetongue (1976) Proc. Am. Assoc. Vet. Lab. Diagn., 19, pp. 361-376Jochim, Pearson, Protocol for the immunodiffusion test for bluetongue (1979) Proc. Am. Assoc. Vet. Lab. Diagn., 22, pp. 463-471Jochim, An overview of diagnostics for bluetongue (1985) B. Jochim, Bluetongue and Related Orbiviruses, pp. 423-433. , Alan R. Liss, New YorkKlontz, Svehag, Gorhan, A study by the agar diffusion technique of precipitating antibody directed against blue tongue virus and its relation to hemotypic neutralizing antibody (1962) Archiv f�r die gesamte Virusforschung, 2, pp. 259-272Knudson, Shope, Overview of the orbiviruses (1985) B. Jochim. Bluetongue and Related Orbiviruses, pp. 255-266. , Alan R. Liss, New YorkLaemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685Matthews, Classification and nomenclature of viruses (1982) Fourth Rep. Int. Com. Tax. Vir. Intervirol., 17, pp. 1-199Mechan, Dean, Jochim, Correlation of serotype specificity and protein structure of the five U.S. serotypes of bluetongue virus (1986) J. Gen. Virol., 67, pp. 2617-2624Ranger, Brown, Bluetongue/epizootic haemorrhagic disease agar gel immunodiffusion (AGID) antigen (1985) NVSL Diagnostic Reagents Production Guide No. R-63/94, pp. 1-4Verwoerd, Els, De, Huismans, Structure of the bluetongue virus capsid (1972) J. Virol., 10, pp. 783-794Verwoerd, Huismans, Studies on the in vitro and the in vivo transcription of the bluetongue virus genome (1972) Onderstepoort J. Vet. Res., 39, pp. 185-192Urakawa, Roy, Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression on the NS1 gene of bluetongue virus serotype 10 (1988) J. Virol., 62, pp. 3919-3927Wang, Luedke, Chow, Soluble antigen of bluetongue virus (1972) Inf. Immunol., 5, pp. 467-47