Immunosurveillance By T-lymphocytes In Pretumoral Stages Of Chemically Induced Pancreatic Carcinogenesis

Cellular immune-response during pancreatic carcinogenesis induced by N-nitroso-bis(2-hydroxy-propyl)amine in Syrian Golden hamsters was studied using a mouse antiserum to hamster T-lymphocytes in indirect immunofluorescence. The chronology of lesions in this model is, acinar cell atypia, cystadenoma, ductal hyperplasia and adenocar-cinoma. Lymphocyte infiltration began before microscopic lesions. Starting as an interstitial and interlobular migration, this earliest population was composed of various kind of mononuclear cells including T-cells. As pancreatic lesions proceeded, an abundant lymphocyte supply through newly formed capillaries (angiogenesis), gave rise to inter- and intralobular nodules composed almost exclusively of T-cells. Migrating from nodules, T-cells invaded and readily destroyed the exocrine tissue. Formation of hyperplastic ducts and of adenocarcinoma was accompanied by considerable accretion of the basal membrane (fibrosis). 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