Real-time Monitoring Of Binding Assays On Microfluidic Devices With Contactless Conductivity Detection

This work describes the development of a dual capacitively coupled contactless conductivity detection (C4D) system and its application to monitor binding assays on microfluidic devices. Microfluidic devices were fabricated in poly(dimethylsiloxane) (PDMS) and sealed against a glass plate containing patterned electrodes. Before the sealing step, the electrodes were insulated from the contact with the microchannel by a thin layer of silicon oxide. The avidin-biotin complex was used as model system to demonstrate the analytical feasibility of the proposed system. © 2008 CBMS.742744Dostalek, J., Homola, J., Surface plasmon resonance sensor based on an array of diffraction gratings for highly parallelized observation of biomolecular interactions (2008) Sens. Actuators B, 129, pp. 303-310Pumera, M., Contactless conductivity detection for microfluidics: Designs and applications (2007) Talanta, 74, pp. 358-364Fracassi Da Silva, J.A., Do Lago, C.L., An oscillometric detector for capillary electrophoresis (1998) Anal. Chem., 70, pp. 4339-434