Isolation And Characterization Of Microsatellite Markers In Acca Sellowiana (berg) Burret

Acca sellowiana has commercial potential because of the quality and the unique flavor of its fruit. Conservation of natural populations and management of breeding programmes would benefit from the availability of molecular markers that could be used to characterize levels and distribution of genetic variability. Thus, 13 microsatellite markers were developed from an enriched genomic library of A. sellowiana. They were characterized using 40 samples. The expected and observed heterozygosities ranged from 0.513 to 0.913 and from 0.200 to 0.889, respectively. These are the first microsatellite loci characterized from A. sellowiana that will contribute to improve researches on the genetic conservation, characterization and breeding.8614171419Billotte, N., Lagoda, P.J.L., Risterucci, A.M., Baurens, F.C., Microsatellite enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288Creste, S., Tulmann-Neto, A., Figueira, A., Detection of single sequence repeat polymorphism in denaturing polyacrylamide sequencing gels by silver staining (2001) Plant Molecular Biology Reporter, 19, pp. 1-8Doyle, J.J., Doyle, J.L., Isolation of plant DNA from fresh tissue (1990) Focus, 12, pp. 13-15Lewis, P.O., Zaykin, D., (2001) Free Program Distributed by the Authors over the Internet, , http://hydrodictyon.eeb.uconn.edu/people/plewis/software.php, Available from URLMattos, J.R., (1990) Goiabeira Serrana Fruteiras Nativas Do Brasil., , 2nd edn. Gráfica Ceue, Porto Alegre, BrazilMellersch, C., Sampson, J., Simplifying detection of microsatellite length polymorphisms (1993) BioTechniques, 15, pp. 582-584Nei, M., Estimation of average heterozigosity and genetic distance from a small number of individuals (1978) Genetics, 89, pp. 583-590Van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M., Shipley, P., Micro-checker: Software for identifying and correcting genotyping errors in microsatellite data (2004) Molecular Ecology Notes, 4, pp. 535-538Rozen, S., Skaletsky, H.J., Primer 3 on the WWW for general users and for biologist programmers (2000) Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365-386. , In: eds. Krawetz, S., Misener, S. pp. Humana Press, Totowa, New JerseySambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual. 2nd Edn., , Cold Spring Harbor Laboratory Press, New YorkSantos, K.L., Welter, L.J., Dantas, A.C.M., Transference of microsatellite markers from Eucalyptus spp. to Acca Sellowiana and the successful use of this technique in genetic characterization (2007) Genetics and Molecular Biology, 30, pp. 73-79Thorp, G., Bieleski, R., (2002) Feijoas: Origins, Cultivation and Uses., , David Bateman and HortResearch, Palmerston North, New Zealan