Characterization Of Reconstructed Human Skin Using Photoacoustic Spectroscopy

Recent progress in skin culture techniques has led to the development of systems in which the reconstructed human skin obtained exhibits morphologic characteristics similar to those observed in vivo. Reconstructed human skin may be the best substrate for pharmacological tests of topically applied drugs; besides, it can be employed in the treatment of burns wounds and chronic skin ulcers. However, this newly developed material must be validated by comparison with human skin, in order to show that reconstructed skin presents characteristics similar to those of human skin. This was accomplished in the present work, through photoacoustic spectroscopy (PAS) measurements. Results show similarity between reconstructed skin and ex-vivo human skin, validating possible therapeutic and cosmetic treatments to be developed using the reconstructed human skin analyzed in this work. © EDP Sciences/ Societé Italiana di Fisica/Springer-Verlag 2008.1531471474Puzzi, M.B., Rehder, J., Souto, L.R.M., Issa, C.M., (2004) Sao Paulo Med. J., 122, p. 22Souto, L.R.M., (2006) Sao Paulo Med. J., 124, p. 71Cario-André, M., (2002) J. Photoch. Photobiol. B, 68, p. 79Rompe, P.C.B., (2005) J. Phys. IV (France), 125, p. 785Oliveira, S., Barja, P.R., (2006) Proceedings of the Environmental and Health World Congress - EHWC 2006, pp. 341-345. , in (COPEC, Santos)Rosencwaig, A., Gersho, A., (1976) J. Appl. Phys., 47, p. 64Kitchen, S., Bazin, S., (1998) Eletroterapia De Clayton, , 10th edn. (São Paulo, Manole)Nicoletti, M.A., (2002) Cosmetics & Toiletries, 14, p. 46Zhang, X., (2000) Photochem. Photobiol., 71, p. 524Bendit, E.G., Ross, D., (1961) Appl. Spectrosc., 15, p. 10