Biogenic Silver Nanoparticles Associated With Silver Chloride Nanoparticles (ag@agcl) Produced By Laccase From Trametes Versicolor

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)In the present study, semi-purified laccase from Trametes versicolor was applied for the synthesis of silver nanoparticles, and the properties of the produced nanoparticles were characterized. All of the analyses of the spectra indicated silver nanoparticle formation. A complete characterization of the silver nanoparticles showed that a complex of silver nanoparticles and silver ions was produced, with the majority of the particles having a Ag2+ chemical structure. A hypothetical mechanistic scheme was proposed, suggesting that the main pathway that was used was the interaction of silver ions with the T1 site of laccase, producing silver nanoparticles with the concomitant inactivation of laccase activity and posterior complexing with silver ions.31CNPq; Fondo Nacional de Desarrollo Científico y Tecnológico; CONICYT; Fondo Nacional de Desarrollo Científico y Tecnológico; FAPESP; Fondo Nacional de Desarrollo Científico y Tecnológico; 1130854; FONDECYT; Fondo Nacional de Desarrollo Científico y TecnológicoFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Acevedo, F., Pizzul, L., Castillo, M.P., González, M.E., Cea, M., Gianfreda, L., Diez, M.C., Degradation of polycyclic aromatic hydrocarbons by free and nanoclay-immobilized manganese peroxidase from Anthracophyllum discolor (2012) Chemosphere, 80, pp. 271-278Ahmad, A., Mukherjee, P., Senapati, S., Mandal, M., Khan, M.I., Kumar, R., Sastry, M., Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum (2003) Colloids Surf B: Biointerf, 28, pp. 313-318. , 10.1016/S0927-7765(02)00174-1Bawaskar, M., Gaikwad, S., Ingle, A., Rathod, D., Gade, A., Durán, N., Marcato, P.D., Rai, M., A new report on mycosynthesis of silver nanoparticles by Fusarium culmorum (2010) Curr Nanosci, 6, pp. 376-380. , 10.2174/157341310791658919Birla, S.S., Gaikwad, S.C., Gade, A.K., Rai, M.K., Rapid synthesis of silver nanoparticles from fusarium oxysporum by optimizing physicocultural conditions (2013) SciWorld J, 2013, p. 796018Cecil, R., McPhee, J.R., Further studies on the reaction of disulphides with silver nitrate (1957) Biochem J, 66, pp. 538-543Chan, Y.S., Don, M.M., Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities (2012) Intern Arabic J Antimicrob Agents, 2N (3), p. 3Chan, Y.S., Don, M.M., Biosynthesis and structural characterization of Ag nanoparticles from white rot fungi (2012) Mat Sci Eng, 33, pp. 282-288Choi, O.K., Deng, K.K., Kim, N.J., Ross, L., Hu, Z.Q., The inhibitory effects of silver nanoparticles, silver ions and silver chloride colloids on microbial growth (2008) Water Res, 42, pp. 3066-3074. , 10.1016/j.watres.2008.02.021Cordi, L., Minussi, R.C., Freire, R.S., Durán, N., Fungal laccase: copper induction, semi-purification, immobilization, phenolic effluent treatment and electrochemical measurement (2007) Afr J Biotechnol, 6, pp. 1255-1259Durán, N., Marcato, P.D., Souza, G., Alves, O.L., Esposito, E., Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment (2007) J Biomed Nanotechnol, 3, pp. 203-208. , 10.1166/jbn.2007.022Durán, N., Marcato, P.D., Durán, M., Yadav, A., Gade, A., Rai, M., Mechanistic aspects in the biogenic synthesis of extracellular metal nanoparticles by peptides, bacteria, fungi and plants (2011) Appl Microbiol Biotechnol, 90, pp. 1609-1624. , 10.1007/s00253-011-3249-8Eby, D.M., Schaeublin, N.M., Farrington, K.E., Hussain, S.M., Johnson, G.R., Lysozyme catalyzes the formation of antimicrobial silver nanoparticles (2009) ACS Nano, 3, pp. 984-994. , 10.1021/nn900079eEdmundson, M.C., Capeness, M., Horsfall, L., Exploring the potential of metallic nanoparticles within synthetic biology (2014) New Biotechnol, , http://dx.doi.org/10.1016/j.nbt.2014.03.004Faramarzi, M.A., Forootanfar, H., Biosynthesis and characterization of gold nanoparticles produced by laccase from Paraconiothyrium variabile (2011) Colloids Surf B: Biointerf, 87, pp. 23-27. , 10.1016/j.colsurfb.2011.04.022Forootanfar, H., Faramarzi, M.A., Shahverdi, A.R., Yazdi, M.T., Purification and biochemical characterization of extracellular laccase from the ascomycete Paraconiothyrium variabile (2011) Bioresour Technol, 102, pp. 1808-1814. , 10.1016/j.biortech.2010.09.043Gade, A., Bonde, P., Ingle, A.P., Marcato, P.D., Durán, N., Rai, M.K.J., Exploitation of Aspergillus niger for synthesis of silver nanoparticles (2008) Biobased Mat Bioenerg, 2, pp. 243-247. , 10.1166/jbmb.2008.401Gaikwad, S.C., Birla, S.S., Ingle, A.P., Gade, A.K., Marcato, P.D., Rai, M., Durán, N.J., Screening of different Fusarium species to select potential species for the synthesis of silver nanoparticles (2013) J Braz Chem Soc, 24, pp. 1974-1982Gopinath, V., Priyadarshini, S., Priyadharsshini, N.M., Pandian, K., Velusamy, P., Biogenic synthesis of antibacterial silver chloride nanoparticles using leaf extracts of Cissus quadrangulares Linn (2013) Mat Lett, 91, pp. 224-227Ingale, A.G., Chaudhari, A.N., Biogenic synthesis of nanoparticles and potential applications: an eco-friendly approach (2013) J Nanomed Nanotechol, 4, p. 165Jain, N., Bhargava, A., Majumdar, S., Tarafdar, J.C., Panwar, J., Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: A mechanism perspective (2011) Nanoscale, 3, pp. 635-641. , 10.1039/c0nr00656dKalishwaralal, K., Gopalram, S., Vaidyanathan, R., Deepak, V., Pandian, S.R.K., Gurunathan, S., Optimization of α-amylase production for the green synthesis of gold nanoparticles (2010) Colloid Surf B Interf, 77, pp. 174-180. , 10.1016/j.colsurfb.2010.01.018Kashyap, P.L., Kumar, S., Kumar Srivastava, A.K., Sharma, A.K., Myconanotechnology in agriculture: a perspective (2013) World J Microbiol Biotechnol, 29, pp. 191-207. , 10.1007/s11274-012-1171-6Kumar, U., Ranjan, A.K., Sharan, C., Hardikarc, A.A., Pundle, A., Poddar, P., Green approach towards size controlled synthesis of biocompatible antibacterial metal nanoparticles in aqueous phase using lysozyme (2012) Curr Nanosci, 8, pp. 130-140. , 10.2174/1573413711208010130Leonowicz, A., Grzywnowicz, K., Quantitative estimation of laccase form in some white-rot fungi using syringaldazine as a substrate (1981) Enzyme Microb Technol, 3, pp. 55-58. , 10.1016/0141-0229(81)90036-3Min, S.H., Yang, J.K., Kim, J.Y., Kwon, Y.U., Development of white antibacterial pigment based on silver chloride nanoparticles and mesoporous silica and its polymer composite (2010) Microporous Mesoporous Mater, 128, pp. 19-25. , 10.1016/j.micromeso.2009.07.020Morozova, O.V., Shumakovich, G.P., Gorbacheva, M.A., Shleev, S.V., Yaropolov, A.I., Blue” laccases (2007) Biochem Mosc, 72, pp. 1136-1150. , 10.1134/S0006297907100112Naik, B., Desai, V., Kowshik, M., Prasad, M.V., Fernando, G.F., Ghosh, N.N., Synthesis of Ag/AgCl–mesoporous silica nanocomposites using a simple aqueous solution-based chemical method and a study of their antibacterial activity on E. coli (2011) Particuology, 9, pp. 243-247. , 10.1016/j.partic.2010.12.001Nithya, R., Ragunathan, R., Synthesis of silver nanoparticle using Pleurotus sajor caju and its antimicrobial study (2009) Digest J Nanomat Biostruct, 4, pp. 623-629Paulkumar, K., Rajeshkumar, S., Gnanajobitha, G., Vanaja, M., Malarkodi, C., Annadurai, G., Biosynthesis of Silver Chloride Nanoparticles Using Bacillus subtilis MTCC 3053 and Assessment of Its Antifungal Activity (2013) ISRN Nanomaterials, 2013 (ID 317963), p. 8. , http://dx.doi.org/10.1155/2013/317963Piontek, C.K., Antorini, M., Choinowski, G.J., Crystal structure of a laccase from the fungus Trametes versicolor at 1.90-Å resolution containing a full complement of coppers (2002) Biol Chem, 277, pp. 37663-37669. , 10.1074/jbc.M204571200Rangnekar, A., Sarma, T.K., Singh, A.K., Deka, J., Ramesh, A., Chattopadhyay, A., Retention of enzymatic activity of α-amylase in the reductive synthesis of gold nanoparticles (1997) Langmuir, 23, pp. 5700-5706Rubilar, O., Diezand, M.C., Gianfreda, L., Transformation of chlorinated phenolic compounds by white rot fungi (2008) Crit Rev Environ Sci Tech, 38, pp. 227-268. , 10.1080/10643380701413351Sanghi, R., Verma, P., Biomimetic synthesis and characterisation of protein capped silver nanoparticles (2009) Bioresour Technol, 100, pp. 501-504. , 10.1016/j.biortech.2008.05.048Sanghi, R., Verma, P., Puri, S., Enzymatic formation of gold nanoparticles using Phanerochaete chrysosporium (2001) Advan Chem Eng Sci, 1, pp. 154-162Sharma, S., Mandani, S., Sarma, T.K., Biogenic growth of alloys and core-shell nanostructures using urease as a nanoreactor at ambient condition (2013) Sci Rep, 3 (2601), pp. 1-8Tortella, G.R., Rubilar, O., Gianfreda, L., Valenzuela, E., Diez, M.C., Enzymatic characterization of Chilean native wood-rotting fungi for potential use in the bioremediation of polluted environments with chlorophenols (2008) World J Microbiol Biotechnol, 12, pp. 2805-2818Tran, Q.H., Nguyen, V.Q., Le, A.T., Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives (2013) Adv Nat Sci: Nanosci Nanotechnol, 4, p. 033001. , 10.1088/2043-6262/4/3/033001Vigneshwaran, N., Kathe, A., Varadarajan, P.V., Nachane, R., Balasubramanya, R.H., Biomimetics of silver nanoparticles by white rot fungus, Phaenerochaete chrysosporium (2009) Colloids Surf B: Biointerf, 53, pp. 55-59Wang, P., Huang, B., Qin, X., Zhang, X., Dai, Y., Wei, J., Whangbo, M.H., Ag@AgCl: A highly efficient and stable photocatalyst active under visible light (2008) Chem Int Ed, 47, pp. 7931-7933. , 10.1002/anie.20080248